Detailed information of TA system TA107100

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1012988..1013905
• Replicon: chromosome
• Accession: NZ_CP030097
• Organism: Bacillus amyloliquefaciens strain SH-B74

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: DOU34_RS05390
• Protein ID: WP_007407256.1
• Coordinates: 1013159..1013905 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: DOU34_RS05385
• Protein ID: WP_003154807.1
• Coordinates: 1012988..1013158 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DOU34_RS05350	1008222..1009844	+	1623	WP_061046843.1	pyocin knob domain-containing protein	-
DOU34_RS05355	1009857..1010228	+	372	WP_039062950.1	YomQ/XkdW protein, phage-like element PBSX	-
DOU34_RS05360	1010233..1010430	+	198	WP_039062951.1	XkdX family protein	-
DOU34_RS05365	1010487..1011248	+	762	WP_039062952.1	hypothetical protein	-
DOU34_RS05370	1011300..1011563	+	264	WP_003154815.1	hemolysin XhlA family protein	-
DOU34_RS05375	1011577..1011840	+	264	WP_003154813.1	phage holin	-
DOU34_RS05380	1011854..1012732	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
DOU34_RS05385	1012988..1013158	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
DOU34_RS05390	1013159..1013905	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
DOU34_RS05395	1014010..1015008	-	999	WP_020955696.1	inorganic phosphate transporter	-
DOU34_RS05400	1015021..1015638	-	618	WP_003154804.1	DUF47 domain-containing protein	-
DOU34_RS05405	1015924..1017240	-	1317	WP_007610842.1	amino acid permease	-
DOU34_RS05410	1017563..1018513	+	951	WP_012117369.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T107100 WP_007407256.1 NZ_CP030097:c1013905-1013159 [Bacillus amyloliquefaciens]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T107100 NZ_CP030097:c1013905-1013159 [Bacillus amyloliquefaciens]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAGATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGATTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT107100 WP_003154807.1 NZ_CP030097:c1013158-1012988 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT107100 NZ_CP030097:c1013158-1012988 [Bacillus amyloliquefaciens]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14