Detailed information of TA system TA107099

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 232118..232755
• Replicon: chromosome
• Accession: NZ_CP030097
• Organism: Bacillus amyloliquefaciens strain SH-B74

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: DOU34_RS01090
• Protein ID: WP_003156187.1
• Coordinates: 232405..232755 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: DOU34_RS01085
• Protein ID: WP_003156188.1
• Coordinates: 232118..232399 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DOU34_RS01065	228483..229082	-	600	WP_061047192.1	rhomboid family intramembrane serine protease	-
DOU34_RS01070	229175..229540	+	366	WP_007410229.1	holo-ACP synthase	-
DOU34_RS01075	229705..230712	+	1008	WP_061047193.1	outer membrane lipoprotein carrier protein LolA	-
DOU34_RS01080	230829..231998	+	1170	WP_061047194.1	alanine racemase	-
DOU34_RS01085	232118..232399	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
DOU34_RS01090	232405..232755	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
DOU34_RS01095	232873..233694	+	822	WP_003156182.1	STAS domain-containing protein	-
DOU34_RS01100	233699..234064	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
DOU34_RS01105	234067..234468	+	402	WP_003156178.1	anti-sigma regulatory factor	-
DOU34_RS01110	234480..235487	+	1008	WP_007410233.1	PP2C family protein-serine/threonine phosphatase	-
DOU34_RS01115	235551..235880	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
DOU34_RS01120	235877..236359	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
DOU34_RS01125	236325..237113	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
DOU34_RS01130	237113..237715	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T107099 WP_003156187.1 NZ_CP030097:232405-232755 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T107099 NZ_CP030097:232405-232755 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT107099 WP_003156188.1 NZ_CP030097:232118-232399 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT107099 NZ_CP030097:232118-232399 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5