Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3658582..3659122 Replicon chromosome
Accession NZ_CP030093
Organism Mycobacterium tuberculosis strain RUS_B0

Toxin (Protein)


Gene name mazF Uniprot ID P9WII0
Locus tag DPM14_RS17940 Protein ID WP_003403376.1
Coordinates 3658814..3659122 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag DPM14_RS17935 Protein ID WP_003403381.1
Coordinates 3658582..3658827 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DPM14_RS17905 3654498..3654671 - 174 WP_003898549.1 hypothetical protein -
DPM14_RS17910 3654668..3655006 - 339 WP_003403405.1 PIN domain-containing protein -
DPM14_RS17915 3654918..3655244 - 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
DPM14_RS17920 3655307..3657670 - 2364 WP_003901895.1 arylsulfatase AtsD -
DPM14_RS17925 3657784..3658038 + 255 WP_003911263.1 antitoxin VapB7 -
DPM14_RS17930 3658035..3658472 + 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin -
DPM14_RS17935 3658582..3658827 + 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
DPM14_RS17940 3658814..3659122 + 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
DPM14_RS17945 3659398..3660114 + 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
DPM14_RS17950 3660190..3660345 + 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
DPM14_RS17955 3660440..3660823 + 384 WP_003403365.1 ribonuclease VapC6 -
DPM14_RS17960 3661211..3662221 - 1011 WP_003911248.1 ABC transporter ATP-binding protein -
DPM14_RS17965 3662302..3663807 - 1506 WP_003403360.1 carotenoid cleavage oxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-101)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11320.10 Da        Isoelectric Point: 11.6554

>T107091 WP_003403376.1 NZ_CP030093:3658814-3659122 [Mycobacterium tuberculosis]
MRRGELWFAATPGGDRPVLVLTRDPVADRIGAVVVVALTRTRRGLVSELELTAVENRVPSDCVVNFDNIHTLPRTAFRRR
ITRLSPARLHEACQTLRASTGC

Download         Length: 309 bp

>T107091 NZ_CP030093:3658814-3659122 [Mycobacterium tuberculosis]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT107091 WP_003403381.1 NZ_CP030093:3658582-3658827 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT107091 NZ_CP030093:3658582-3658827 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSE5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References