Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3182647..3183278 Replicon chromosome
Accession NZ_CP030093
Organism Mycobacterium tuberculosis strain RUS_B0

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag DPM14_RS15430 Protein ID WP_003405820.1
Coordinates 3182967..3183278 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag DPM14_RS15425 Protein ID WP_003405836.1
Coordinates 3182647..3182967 (+) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DPM14_RS15400 3177843..3178481 + 639 WP_003898730.1 lipid droplet-associated protein -
DPM14_RS15405 3178478..3179725 + 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
DPM14_RS15410 3179715..3179972 + 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
DPM14_RS15415 3179982..3181094 + 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
DPM14_RS15420 3181112..3182637 - 1526 Protein_2920 carboxylesterase/lipase family protein -
DPM14_RS15425 3182647..3182967 + 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
DPM14_RS15430 3182967..3183278 + 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
DPM14_RS15435 3183390..3184547 + 1158 WP_003898727.1 AI-2E family transporter -
DPM14_RS15440 3184554..3185198 - 645 WP_003915494.1 DUF4245 domain-containing protein -
DPM14_RS15445 3185254..3186342 + 1089 WP_003898726.1 class II fructose-bisphosphatase -
DPM14_RS15450 3186373..3187797 + 1425 WP_003405805.1 class II fumarate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T107085 WP_003405820.1 NZ_CP030093:3182967-3183278 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T107085 NZ_CP030093:3182967-3183278 [Mycobacterium tuberculosis]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT107085 WP_003405836.1 NZ_CP030093:3182647-3182967 [Mycobacterium tuberculosis]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT107085 NZ_CP030093:3182647-3182967 [Mycobacterium tuberculosis]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References