Detailed information of TA system TA107084

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3173954..3174522
• Replicon: chromosome
• Accession: NZ_CP030093
• Organism: Mycobacterium tuberculosis strain RUS_B0

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: DPM14_RS15375
• Protein ID: WP_003405865.1
• Coordinates: 3173954..3174328 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: DPM14_RS15380
• Protein ID: WP_003405863.1
• Coordinates: 3174325..3174522 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DPM14_RS15345	3170367..3171074	+	708	Protein_2904	adenylate/guanylate cyclase domain-containing protein	-
DPM14_RS21910	3171026..3171235	-	210	WP_003911400.1	hypothetical protein	-
DPM14_RS15350	3171257..3171967	+	711	WP_003911399.1	hypothetical protein	-
DPM14_RS15355	3171982..3172305	-	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
DPM14_RS15360	3172548..3172823	+	276	WP_003405867.1	hypothetical protein	-
DPM14_RS15365	3172750..3172935	-	186	WP_003901093.1	hypothetical protein	-
DPM14_RS15370	3173053..3173751	-	699	WP_003898733.1	hypothetical protein	-
DPM14_RS15375	3173954..3174328	-	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
DPM14_RS15380	3174325..3174522	-	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
DPM14_RS15385	3174610..3175683	-	1074	WP_003898732.1	redox-regulated ATPase YchF	-
DPM14_RS15390	3175746..3176729	+	984	WP_003898731.1	hypothetical protein	-
DPM14_RS15395	3176746..3177753	-	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
DPM14_RS15400	3177843..3178481	+	639	WP_003898730.1	lipid droplet-associated protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T107084 WP_003405865.1 NZ_CP030093:c3174328-3173954 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T107084 NZ_CP030093:c3174328-3173954 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT107084 WP_003405863.1 NZ_CP030093:c3174522-3174325 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT107084 NZ_CP030093:c3174522-3174325 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07