Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2884875..2885445 Replicon chromosome
Accession NZ_CP030093
Organism Mycobacterium tuberculosis strain RUS_B0

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag DPM14_RS14065 Protein ID WP_003414166.1
Coordinates 2884875..2885231 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag DPM14_RS14070 Protein ID WP_003901465.1
Coordinates 2885215..2885445 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DPM14_RS14045 2880327..2882015 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
DPM14_RS14050 2882019..2882345 - 327 WP_003414157.1 hypothetical protein -
DPM14_RS14055 2882518..2883105 + 588 WP_003914429.1 DUF3558 family protein -
DPM14_RS14060 2883124..2884773 + 1650 Protein_2661 CocE/NonD family hydrolase -
DPM14_RS14065 2884875..2885231 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
DPM14_RS14070 2885215..2885445 - 231 WP_003901465.1 antitoxin MazE Antitoxin
DPM14_RS14075 2885488..2886531 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
DPM14_RS14080 2886722..2886997 + 276 WP_003911993.1 DUF1778 domain-containing protein -
DPM14_RS21650 2887173..2887427 - 255 WP_003917684.1 hypothetical protein -
DPM14_RS14090 2887575..2887979 + 405 WP_003414181.1 hypothetical protein -
DPM14_RS14095 2887976..2888167 + 192 WP_003414184.1 hypothetical protein -
DPM14_RS14100 2888231..2889520 + 1290 Protein_2669 transposase family protein -
DPM14_RS14105 2889754..2890011 + 258 WP_003899489.1 hypothetical protein -
DPM14_RS14110 2890116..2890427 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T107079 WP_003414166.1 NZ_CP030093:c2885231-2884875 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T107079 NZ_CP030093:c2885231-2884875 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT107079 WP_003901465.1 NZ_CP030093:c2885445-2885215 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT107079 NZ_CP030093:c2885445-2885215 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References