Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2644186..2644823 Replicon chromosome
Accession NZ_CP030093
Organism Mycobacterium tuberculosis strain RUS_B0

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag DPM14_RS12705 Protein ID WP_003413180.1
Coordinates 2644186..2644581 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag DPM14_RS12710 Protein ID WP_003413183.1
Coordinates 2644578..2644823 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DPM14_RS12660 2639590..2640801 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
DPM14_RS12670 2640928..2641587 + 660 WP_003902296.1 hypothetical protein -
DPM14_RS12675 2641584..2642246 + 663 WP_003905878.1 hypothetical protein -
DPM14_RS12680 2642243..2642520 + 278 Protein_2399 type II toxin-antitoxin system VapB family antitoxin -
DPM14_RS12685 2642613..2643026 + 414 WP_003413164.1 PIN domain nuclease -
DPM14_RS12690 2643065..2643322 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
DPM14_RS12695 2643319..2643696 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
DPM14_RS12700 2643712..2644086 - 375 WP_003413177.1 hypothetical protein -
DPM14_RS12705 2644186..2644581 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
DPM14_RS12710 2644578..2644823 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
DPM14_RS12720 2645234..2645653 - 420 WP_003413190.1 A24 family peptidase -
DPM14_RS12725 2645665..2646474 - 810 WP_003413193.1 shikimate dehydrogenase -
DPM14_RS12730 2646471..2647724 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
DPM14_RS12735 2647717..2648229 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T107074 WP_003413180.1 NZ_CP030093:c2644581-2644186 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T107074 NZ_CP030093:c2644581-2644186 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT107074 WP_003413183.1 NZ_CP030093:c2644823-2644578 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT107074 NZ_CP030093:c2644823-2644578 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References