Detailed information of TA system TA107073

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2643065..2643696
• Replicon: chromosome
• Accession: NZ_CP030093
• Organism: Mycobacterium tuberculosis strain RUS_B0

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: DPM14_RS12695
• Protein ID: WP_003413174.1
• Coordinates: 2643319..2643696 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: DPM14_RS12690
• Protein ID: WP_003413167.1
• Coordinates: 2643065..2643322 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DPM14_RS12655	2638980..2639294	+	315	WP_009937839.1	hypothetical protein	-
DPM14_RS12660	2639590..2640801	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
DPM14_RS12670	2640928..2641587	+	660	WP_003902296.1	hypothetical protein	-
DPM14_RS12675	2641584..2642246	+	663	WP_003905878.1	hypothetical protein	-
DPM14_RS12680	2642243..2642520	+	278	Protein_2399	type II toxin-antitoxin system VapB family antitoxin	-
DPM14_RS12685	2642613..2643026	+	414	WP_003413164.1	PIN domain nuclease	-
DPM14_RS12690	2643065..2643322	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
DPM14_RS12695	2643319..2643696	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
DPM14_RS12700	2643712..2644086	-	375	WP_003413177.1	hypothetical protein	-
DPM14_RS12705	2644186..2644581	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
DPM14_RS12710	2644578..2644823	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
DPM14_RS12720	2645234..2645653	-	420	WP_003413190.1	A24 family peptidase	-
DPM14_RS12725	2645665..2646474	-	810	WP_003413193.1	shikimate dehydrogenase	-
DPM14_RS12730	2646471..2647724	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
DPM14_RS12735	2647717..2648229	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T107073 WP_003413174.1 NZ_CP030093:2643319-2643696 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T107073 NZ_CP030093:2643319-2643696 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT107073 WP_003413167.1 NZ_CP030093:2643065-2643322 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT107073 NZ_CP030093:2643065-2643322 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5