Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2004129..2004715 Replicon chromosome
Accession NZ_CP030093
Organism Mycobacterium tuberculosis strain RUS_B0

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag DPM14_RS09585 Protein ID WP_003410010.1
Coordinates 2004129..2004473 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag DPM14_RS09590 Protein ID WP_003410014.1
Coordinates 2004467..2004715 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DPM14_RS09545 1999835..2000434 + 600 WP_003910883.1 L-lysine exporter -
DPM14_RS09550 2000850..2001278 + 429 WP_003409992.1 cellulose-binding protein -
DPM14_RS09555 2001504..2002043 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
DPM14_RS09565 2002563..2003123 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
DPM14_RS09570 2003120..2003461 - 342 WP_003410003.1 DUF2384 domain-containing protein -
DPM14_RS09575 2003547..2003804 + 258 WP_003410006.1 hypothetical protein -
DPM14_RS09580 2003705..2004040 - 336 WP_003410009.1 dehydrogenase -
DPM14_RS09585 2004129..2004473 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
DPM14_RS09590 2004467..2004715 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
DPM14_RS09595 2004815..2007130 - 2316 WP_003899120.1 cation transporter ATPase CptG -
DPM14_RS09600 2007127..2007399 - 273 WP_003410017.1 DUF1490 family protein -
DPM14_RS09605 2007452..2007808 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
DPM14_RS09610 2007965..2008731 + 767 Protein_1823 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T107064 WP_003410010.1 NZ_CP030093:c2004473-2004129 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T107064 NZ_CP030093:c2004473-2004129 [Mycobacterium tuberculosis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT107064 WP_003410014.1 NZ_CP030093:c2004715-2004467 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT107064 NZ_CP030093:c2004715-2004467 [Mycobacterium tuberculosis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References