Detailed information of TA system TA107059

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1961728..1962431
• Replicon: chromosome
• Accession: NZ_CP030093
• Organism: Mycobacterium tuberculosis strain RUS_B0

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: DPM14_RS09260
• Protein ID: WP_003409778.1
• Coordinates: 1961728..1962057 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: DPM14_RS09265
• Protein ID: WP_003409780.1
• Coordinates: 1962054..1962431 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DPM14_RS09240	1958111..1959181	+	1071	WP_003902252.1	epoxide hydrolase EphB	-
DPM14_RS09245	1959178..1959693	+	516	WP_003409718.1	flavin reductase family protein	-
DPM14_RS09250	1959690..1960751	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
DPM14_RS09255	1960748..1961518	+	771	WP_003409775.1	SDR family oxidoreductase	-
DPM14_RS09260	1961728..1962057	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DPM14_RS09265	1962054..1962431	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
DPM14_RS09270	1962428..1963018	-	591	WP_003409784.1	SEC-C domain-containing protein	-
DPM14_RS09275	1963073..1964437	+	1365	WP_003910878.1	HNH endonuclease	-
DPM14_RS09280	1964592..1965044	-	453	WP_003899095.1	lipoprotein	-
DPM14_RS09285	1965108..1965509	+	402	WP_003409869.1	hypothetical protein	-
DPM14_RS09290	1965502..1965684	-	183	WP_003409870.1	hypothetical protein	-
DPM14_RS09295	1965798..1966148	-	351	WP_003409871.1	hypothetical protein	-
DPM14_RS09300	1966159..1967061	-	903	WP_003899097.1	hypothetical protein	-
DPM14_RS09305	1967082..1967273	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T107059 WP_003409778.1 NZ_CP030093:c1962057-1961728 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T107059 NZ_CP030093:c1962057-1961728 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT107059 WP_003409780.1 NZ_CP030093:c1962431-1962054 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT107059 NZ_CP030093:c1962431-1962054 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8