Detailed information of TA system TA107048

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 701479..702122
• Replicon: chromosome
• Accession: NZ_CP030093
• Organism: Mycobacterium tuberculosis strain RUS_B0

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: DPM14_RS03240
• Protein ID: WP_003403187.1
• Coordinates: 701721..702122 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ91
• Locus tag: DPM14_RS03235
• Protein ID: WP_003403184.1
• Coordinates: 701479..701724 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DPM14_RS03195	696759..697289	-	531	WP_003917320.1	two component system sensor kinase HK2	-
DPM14_RS03200	697273..697968	-	696	WP_003918099.1	two-component system response regulator TcrA	-
DPM14_RS03205	698091..698402	+	312	WP_003403164.1	hypothetical protein	-
DPM14_RS03210	698474..699424	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
DPM14_RS03220	699665..700249	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
DPM14_RS03225	700251..700964	+	714	Protein_623	IS607 family element transposase accessory protein TnpB	-
DPM14_RS03230	700964..701434	+	471	WP_003898523.1	hypothetical protein	-
DPM14_RS03235	701479..701724	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
DPM14_RS03240	701721..702122	+	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
DPM14_RS03255	702478..703248	+	771	Protein_627	IS3-like element IS987 family transposase	-
DPM14_RS03260	703356..703577	+	222	WP_003906064.1	hypothetical protein	-
DPM14_RS03265	703672..704817	-	1146	WP_003417299.1	FHA domain-containing protein	-
DPM14_RS03280	705462..706526	+	1065	WP_003913037.1	GTP 3',8-cyclase MoaA	-
DPM14_RS03285	706623..706997	+	375	WP_003417295.1	4a-hydroxytetrahydrobiopterin dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	699665..703248	3583

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T107048 WP_003403187.1 NZ_CP030093:701721-702122 [Mycobacterium tuberculosis]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T107048 NZ_CP030093:701721-702122 [Mycobacterium tuberculosis]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT107048 WP_003403184.1 NZ_CP030093:701479-701724 [Mycobacterium tuberculosis]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT107048 NZ_CP030093:701479-701724 [Mycobacterium tuberculosis]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6

Antitoxin

• AlphaFold DB: G0TQ91