Detailed information of TA system TA107036

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4273757..4274796
• Replicon: chromosome
• Accession: NZ_CP030092
• Organism: Massilia sp. YMA4

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: DPH57_RS18300
• Protein ID: WP_112939033.1
• Coordinates: 4273757..4274332 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: DPH57_RS18305
• Protein ID: WP_112939034.1
• Coordinates: 4274329..4274796 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DPH57_RS18285	4269796..4271160	-	1365	WP_112939030.1	beta-glucosidase	-
DPH57_RS18290	4271147..4272442	-	1296	WP_112939031.1	cellulase family glycosylhydrolase	-
DPH57_RS18295	4272527..4273561	-	1035	WP_112939032.1	LacI family DNA-binding transcriptional regulator	-
DPH57_RS18300	4273757..4274332	-	576	WP_112939033.1	PIN domain-containing protein	Toxin
DPH57_RS18305	4274329..4274796	-	468	WP_112939034.1	helix-turn-helix domain-containing protein	Antitoxin
DPH57_RS18310	4274907..4276361	-	1455	WP_112939035.1	NAD(P)(+) transhydrogenase (Re/Si-specific) subunit beta	-
DPH57_RS18315	4276361..4276666	-	306	WP_112939036.1	NAD(P) transhydrogenase subunit alpha	-
DPH57_RS18320	4276681..4277793	-	1113	WP_112939037.1	Re/Si-specific NAD(P)(+) transhydrogenase subunit alpha	-
DPH57_RS18325	4278048..4278527	+	480	WP_112939038.1	NUDIX hydrolase	-
DPH57_RS18330	4278545..4279660	+	1116	WP_112939039.1	tRNA 2-thiouridine(34) synthase MnmA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-115)

Antitoxin

• SinI-like, DNA-binding domain (84-130)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21707.13 Da
• Isoelectric Point: 6.4772

>T107036 WP_112939033.1 NZ_CP030092:c4274332-4273757 [Massilia sp. YMA4]
MRISPFTVVYDACVLYPAPLRDFLMWLALSGCFSARWSERIHGEWKRNLLKNRPDLMQAQLDRTSALMDAAVPDCLVRGY
EKLMPGLTLPDPDDRHVLAAAIRCGASVIVTFNVRDFPAEALREFAIEAQHPDEFTENLFDLDREAVLAAARHQRVQLSN
PAIGIERYLDILRRQGLPRTVDALAVYRCLL

Nucleotide

• Length: 576 bp

>T107036 NZ_CP030092:c4274332-4273757 [Massilia sp. YMA4]
ATGAGAATCTCCCCCTTCACCGTGGTCTACGACGCTTGCGTGCTGTATCCGGCACCGCTGCGCGACTTCCTGATGTGGCT
GGCACTGTCCGGCTGCTTCAGTGCGCGCTGGAGCGAACGTATTCATGGTGAATGGAAGCGCAACCTGCTGAAGAATCGCC
CCGACCTGATGCAGGCCCAGCTCGATCGCACGTCCGCGCTGATGGATGCAGCGGTACCGGACTGCCTGGTGCGCGGCTAC
GAGAAGTTGATGCCGGGTCTTACCCTGCCCGACCCGGACGACCGCCACGTGCTGGCGGCGGCGATTCGCTGCGGCGCCAG
CGTCATCGTCACCTTCAATGTGCGCGATTTCCCGGCCGAGGCGCTGCGCGAATTTGCCATCGAGGCACAGCATCCGGACG
AATTCACCGAAAACCTGTTCGACCTCGATCGCGAGGCCGTGCTGGCCGCAGCACGCCACCAGCGCGTTCAGCTGAGCAAT
CCCGCGATTGGCATCGAACGCTACCTCGACATCCTGCGCCGCCAGGGGCTACCCCGCACCGTGGACGCCTTGGCGGTGTA
CCGCTGCCTGCTCTGA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 16698.16 Da
• Isoelectric Point: 6.7737

>AT107036 WP_112939034.1 NZ_CP030092:c4274796-4274329 [Massilia sp. YMA4]
MSTTVDFTLELPAAAEIQAALQGQRVLAAHLATTHPTQSIQIVDSAGQPHQVELPTSALRLLGDVLAELAAGNAVRVVPV
HAELTTQDAADLLNVSRPHLVKLLEEGALPFHRAGKHRRVRFADLMQFKAAREAASHHAMKALAEQALELKLGYE

Nucleotide

• Length: 468 bp

>AT107036 NZ_CP030092:c4274796-4274329 [Massilia sp. YMA4]
ATGTCCACCACCGTAGATTTCACTTTGGAATTGCCGGCCGCGGCGGAAATCCAGGCCGCGCTGCAGGGCCAGCGCGTGCT
TGCCGCGCACTTGGCGACGACCCATCCTACCCAATCGATTCAGATCGTCGATAGCGCCGGGCAGCCGCATCAAGTCGAGC
TTCCCACGAGCGCCTTGCGCCTGCTGGGCGACGTGCTGGCGGAACTGGCGGCCGGCAATGCCGTCCGGGTGGTGCCGGTG
CACGCCGAGCTGACCACGCAGGATGCGGCCGACCTGCTCAATGTATCGCGCCCGCACCTCGTCAAGCTGCTGGAGGAGGG
CGCATTGCCGTTTCATCGAGCGGGCAAGCACCGGAGAGTTCGGTTCGCCGACCTGATGCAGTTCAAGGCTGCGCGCGAAG
CGGCAAGCCATCATGCAATGAAAGCGCTGGCGGAACAGGCGCTGGAACTCAAACTTGGCTATGAATGA