Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 190172..190698 Replicon plasmid pIMP-20710
Accession NZ_CP030080
Organism Enterobacter hormaechei strain 20710

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag DPF84_RS27325 Protein ID WP_000323025.1
Coordinates 190411..190698 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag DPF84_RS27320 Protein ID WP_000534858.1
Coordinates 190172..190411 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DPF84_RS27300 186500..187738 + 1239 WP_000219087.1 IS110 family transposase -
DPF84_RS27305 188214..188786 + 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
DPF84_RS27310 188986..189909 + 924 WP_000167917.1 cation diffusion facilitator family transporter -
DPF84_RS27320 190172..190411 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
DPF84_RS27325 190411..190698 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
DPF84_RS27330 190770..190928 + 159 WP_001447866.1 type I toxin-antitoxin system Hok family toxin -
DPF84_RS27335 191537..191857 - 321 WP_000332796.1 hypothetical protein -
DPF84_RS27340 192139..192342 - 204 WP_001015183.1 hypothetical protein -
DPF84_RS27345 192389..192739 - 351 WP_000743059.1 hypothetical protein -
DPF84_RS27350 192799..193401 - 603 WP_012695474.1 hypothetical protein -
DPF84_RS27355 193497..194441 - 945 WP_000778029.1 DUF5417 domain-containing protein -
DPF84_RS28460 194952..195128 + 177 WP_001371930.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid formA / mcr-9 / dfrA12 / aadA2 / qacE / floR / tet(G) / blaIMP-1 - 1..286652 286652
- flank IS/Tn - - 184999..185967 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T107028 WP_000323025.1 NZ_CP030080:190411-190698 [Enterobacter hormaechei]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T107028 NZ_CP030080:190411-190698 [Enterobacter hormaechei]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT107028 WP_000534858.1 NZ_CP030080:190172-190411 [Enterobacter hormaechei]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT107028 NZ_CP030080:190172-190411 [Enterobacter hormaechei]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References