Detailed information of TA system TA107026

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/FR47-DUF1778
• Location: 35903..36700
• Replicon: plasmid p4-20710
• Accession: NZ_CP030078
• Organism: Enterobacter hormaechei strain 20710

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: F5S3R9
• Locus tag: DPF84_RS25350
• Protein ID: WP_006812498.1
• Coordinates: 36176..36700 (+)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: F5S3R8
• Locus tag: DPF84_RS25345
• Protein ID: WP_006812497.1
• Coordinates: 35903..36172 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DPF84_RS25315	31293..32633	-	1341	WP_006812582.1	AAA family ATPase	-
DPF84_RS25320	32694..33419	-	726	WP_100229815.1	hypothetical protein	-
DPF84_RS25325	33684..34481	+	798	WP_080395882.1	DUF2971 domain-containing protein	-
DPF84_RS25330	34522..34875	-	354	WP_160859104.1	hypothetical protein	-
DPF84_RS25335	34881..35546	-	666	WP_023315962.1	AAA family ATPase	-
DPF84_RS25345	35903..36172	+	270	WP_006812497.1	DUF1778 domain-containing protein	Antitoxin
DPF84_RS25350	36176..36700	+	525	WP_006812498.1	GNAT family N-acetyltransferase	Toxin
DPF84_RS28430	36727..36933	-	207	WP_023316023.1	hypothetical protein	-
DPF84_RS28435	36884..37135	-	252	WP_042863552.1	hypothetical protein	-
DPF84_RS25360	37137..37829	-	693	WP_006812500.1	membrane protein	-
DPF84_RS25365	37843..38166	-	324	WP_042863551.1	hypothetical protein	-
DPF84_RS25370	38241..38336	-	96	Protein_47	receptor-recognizing protein	-
DPF84_RS25375	38311..39012	-	702	Protein_48	phage tail sheath subtilisin-like domain-containing protein	-
DPF84_RS25380	39387..39818	-	432	WP_042863549.1	tail fiber assembly protein	-
DPF84_RS25385	39820..40365	-	546	Protein_50	phage tail protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..108358	108358

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19356.13 Da
• Isoelectric Point: 8.9844

>T107026 WP_006812498.1 NZ_CP030078:36176-36700 [Enterobacter hormaechei]
VANLTIEMFSEEAVYDFSCFDCGETSLNDFLNNRLAQQHSGRILRGYLLLTRDPIPKVMGFYTLSGSCFARNTLPSNTQQ
RKVPYSDAPSVTLGRLAIDKSIQRQGYGETLVAHAMKVVYRASLAVGIYALFVDAKNENAMRFYKQLGFTPLTGDNANSL
FYPTKGIEKLFGGK

Nucleotide

• Length: 525 bp

>T107026 NZ_CP030078:36176-36700 [Enterobacter hormaechei]
GTGGCCAACCTGACCATTGAGATGTTTTCAGAAGAGGCTGTATATGATTTCTCATGCTTCGACTGCGGAGAAACGTCTCT
CAATGATTTTCTTAACAATCGCCTCGCCCAGCAACACAGCGGGCGTATATTACGCGGATATCTGCTTCTGACGAGAGATC
CGATACCGAAAGTGATGGGGTTTTACACGTTGTCAGGGAGCTGCTTTGCAAGAAACACGCTTCCTTCCAACACTCAACAG
CGTAAAGTCCCCTATTCCGACGCCCCAAGCGTTACGCTCGGTCGTCTGGCAATAGACAAAAGTATCCAGCGACAAGGGTA
TGGGGAAACGCTGGTGGCACATGCGATGAAAGTGGTATACCGGGCTTCCCTGGCCGTTGGGATCTACGCTTTGTTTGTTG
ACGCCAAAAATGAGAATGCGATGCGGTTTTATAAGCAGTTAGGCTTTACCCCTCTTACGGGTGATAACGCCAATTCGCTG
TTCTATCCCACAAAAGGCATTGAGAAGTTGTTTGGTGGAAAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10114.51 Da
• Isoelectric Point: 4.8483

>AT107026 WP_006812497.1 NZ_CP030078:35903-36172 [Enterobacter hormaechei]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNIVMDAISNPPAPNDRLKR
AAERLQSME

Nucleotide

• Length: 270 bp

>AT107026 NZ_CP030078:35903-36172 [Enterobacter hormaechei]
ATGTCAGCACTGAAAAAACAGCGCATCGATCTCAGATTAACCGACGACGACAAGAGCATGATCGAAGAAGCTGCGGCAAT
GACCAACCAGACAATTACTCAGTTTATGGTTGCCAGTGCCTCTGAACGTGCTGCGGAAGTGATAGAGCAACATCGTCGCC
TGATTCTTAGCGAAGAGTCATGGAATATAGTGATGGATGCCATCAGTAACCCGCCTGCACCGAATGATAGGCTGAAACGA
GCTGCCGAGCGTCTACAAAGCATGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A2J0QWY8

Antitoxin

• AlphaFold DB: A0A2J0QUA3