Detailed information of TA system TA107009

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 1319607..1320310
• Replicon: chromosome
• Accession: NZ_CP030076
• Organism: Enterobacter hormaechei strain 20710

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: DPF84_RS06660
• Protein ID: WP_040196446.1
• Coordinates: 1319607..1319948 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: DPF84_RS06665
• Protein ID: WP_040196448.1
• Coordinates: 1319969..1320310 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DPF84_RS06625	1314607..1314954	+	348	WP_045345958.1	hypothetical protein	-
DPF84_RS06630	1315005..1315502	-	498	WP_023315311.1	type VI secretion system tube protein Hcp	-
DPF84_RS06635	1315521..1315853	-	333	WP_045345957.1	DUF1493 family protein	-
DPF84_RS06640	1315847..1316305	-	459	WP_045345955.1	hypothetical protein	-
DPF84_RS06645	1316522..1316989	-	468	WP_072203119.1	SymE family type I addiction module toxin	-
DPF84_RS06650	1317290..1318156	+	867	WP_045339567.1	HNH endonuclease	-
DPF84_RS06655	1318341..1319348	-	1008	WP_044864458.1	restriction endonuclease	-
DPF84_RS06660	1319607..1319948	-	342	WP_040196446.1	TA system toxin CbtA family protein	Toxin
DPF84_RS06665	1319969..1320310	-	342	WP_040196448.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DPF84_RS06670	1320321..1320863	-	543	WP_112777525.1	DNA repair protein RadC	-
DPF84_RS06675	1320876..1321319	-	444	WP_112777524.1	antirestriction protein	-
DPF84_RS06680	1321350..1322171	-	822	WP_112777523.1	DUF945 domain-containing protein	-
DPF84_RS06685	1322270..1322500	-	231	WP_112777522.1	DUF905 domain-containing protein	-
DPF84_RS06690	1322572..1323021	-	450	WP_014226752.1	hypothetical protein	-
DPF84_RS06695	1323018..1323470	-	453	WP_112777521.1	hypothetical protein	-
DPF84_RS06700	1323507..1324076	-	570	WP_112777520.1	hypothetical protein	-
DPF84_RS06705	1324076..1324780	-	705	WP_049190322.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	fosA	-	1312803..1345810	33007

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12791.74 Da
• Isoelectric Point: 7.1648

>T107009 WP_040196446.1 NZ_CP030076:c1319948-1319607 [Enterobacter hormaechei]
MKTLPATTPQAVTLCLSPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYELVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLKRNRISAAQ

Nucleotide

• Length: 342 bp

>T107009 NZ_CP030076:c1319948-1319607 [Enterobacter hormaechei]
ATGAAAACTCTACCTGCAACAACGCCGCAGGCGGTGACGCTATGTCTGTCGCCCGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGAACTGGTTCGTATTGATCGTAGGGGATTC
AACTGGCAAGAACAGTCCCCATATCTGCGGGCAGTCGATATTCTGCGGGCGCGGCAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACAGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12829.33 Da
• Isoelectric Point: 5.4792

>AT107009 WP_040196448.1 NZ_CP030076:c1320310-1319969 [Enterobacter hormaechei]
MKSTDSENDSFLQWGLNRNVTPCFGARLVQEGNRLHYLVDRASITGDFSNAESLKLDEVFPHFISQMESMLTTGEMNPRH
AHCVTLYHNGFTCEADTLGSYGYVYIAIYPTQR

Nucleotide

• Length: 342 bp

>AT107009 NZ_CP030076:c1320310-1319969 [Enterobacter hormaechei]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCCAGTGGGGGCTGAATCGTAATGTGACGCCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGTTGACCGGGCCAGCATTACCGGTGACTTCAGTAACGCAGAATCTT
TGAAGTTGGATGAAGTATTCCCACATTTTATCAGCCAGATGGAGTCGATGTTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CATTTACCCCACTCAACGTTAA