Detailed information of TA system TA106973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 43020..43645
• Replicon: chromosome
• Accession: NZ_CP030070
• Organism: Klebsiella pneumoniae strain IA565

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: R4Y4A3
• Locus tag: DPF99_RS02380
• Protein ID: WP_002882817.1
• Coordinates: 43262..43645 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: DPF99_RS02375
• Protein ID: WP_162557435.1
• Coordinates: 43020..43262 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DPF99_RS02350	39010..39609	+	600	WP_004151865.1	glucose-1-phosphatase	-
DPF99_RS02355	39603..40463	+	861	WP_032445466.1	virulence factor BrkB family protein	-
DPF99_RS02360	40460..40897	+	438	WP_002882809.1	D-tyrosyl-tRNA(Tyr) deacylase	-
DPF99_RS02365	40942..41883	+	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
DPF99_RS02370	41898..42815	-	918	WP_032417884.1	alpha/beta hydrolase	-
DPF99_RS02375	43020..43262	+	243	WP_162557435.1	ribbon-helix-helix domain-containing protein	Antitoxin
DPF99_RS02380	43262..43645	+	384	WP_002882817.1	type II toxin-antitoxin system VapC family toxin	Toxin
DPF99_RS02385	43819..44748	-	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
DPF99_RS02390	44745..45380	-	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
DPF99_RS02395	45377..46279	-	903	WP_002882822.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T106973 WP_002882817.1 NZ_CP030070:43262-43645 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T106973 NZ_CP030070:43262-43645 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9557.79 Da
• Isoelectric Point: 4.3509

>AT106973 WP_162557435.1 NZ_CP030070:43020-43262 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNISRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT106973 NZ_CP030070:43020-43262 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTTCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A0H3GPK8