Detailed information of TA system TA106972

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 26376..26901
• Replicon: plasmid pDA11912.2
• Accession: NZ_CP030067
• Organism: Klebsiella pneumoniae strain IA565

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D4HQE7
• Locus tag: DPF99_RS01060
• Protein ID: WP_013023785.1
• Coordinates: 26596..26901 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: DPF99_RS01055
• Protein ID: WP_004197642.1
• Coordinates: 26376..26594 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DPF99_RS01020	21628..21831	-	204	WP_072010199.1	hemolysin expression modulator Hha	-
DPF99_RS01025	21903..22871	+	969	WP_075211999.1	IS5 family transposase	-
DPF99_RS01030	23063..23743	-	681	WP_032445676.1	hypothetical protein	-
DPF99_RS01035	24037..25065	-	1029	WP_032445668.1	Abi family protein	-
DPF99_RS01040	25226..25807	-	582	WP_032445670.1	hypothetical protein	-
DPF99_RS01055	26376..26594	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
DPF99_RS01060	26596..26901	+	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	Toxin
DPF99_RS01065	27088..28092	+	1005	WP_032445672.1	hypothetical protein	-
DPF99_RS01070	28290..29084	+	795	WP_048991183.1	site-specific integrase	-
DPF99_RS27375	29171..29368	-	198	WP_032445779.1	hypothetical protein	-
DPF99_RS01075	29532..29711	-	180	WP_006788217.1	hypothetical protein	-
DPF99_RS01080	29831..30457	-	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..97903	97903

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11615.30 Da
• Isoelectric Point: 6.4672

>T106972 WP_013023785.1 NZ_CP030067:26596-26901 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T106972 NZ_CP030067:26596-26901 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTATATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT106972 WP_004197642.1 NZ_CP030067:26376-26594 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT106972 NZ_CP030067:26376-26594 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A2J4ZZW6