Detailed information of TA system TA106971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 92207..92729
• Replicon: plasmid pDA11912.1
• Accession: NZ_CP030066
• Organism: Klebsiella pneumoniae strain IA565

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: -
• Locus tag: DPF99_RS00500
• Protein ID: WP_145778088.1
• Coordinates: 92445..92729 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: DPF99_RS00495
• Protein ID: WP_004187025.1
• Coordinates: 92207..92455 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DPF99_RS00465	89574..90143	-	570	WP_004152117.1	small heat shock protein sHSP20	-
DPF99_RS00470	90178..90459	-	282	WP_013307885.1	helix-turn-helix domain-containing protein	-
DPF99_RS00475	90703..90966	-	264	WP_004118208.1	hypothetical protein	-
DPF99_RS00480	90981..91244	+	264	WP_004187027.1	transposase	-
DPF99_RS00485	91189..91305	+	117	Protein_90	transposase domain-containing protein	-
DPF99_RS00490	91493..91917	+	425	Protein_91	IS1 family transposase	-
DPF99_RS00495	92207..92455	+	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
DPF99_RS00500	92445..92729	+	285	WP_145778088.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DPF99_RS00505	92745..94100	-	1356	WP_004187017.1	ISNCY family transposase	-
DPF99_RS27365	94182..94481	-	300	Protein_95	conjugal transfer protein TraH	-
DPF99_RS27370	94479..94962	-	484	Protein_96	hypothetical protein	-
DPF99_RS00515	94979..95503	-	525	Protein_97	hypothetical protein	-
DPF99_RS00525	97046..97510	+	465	WP_004187010.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mrkA / mrkB / mrkC / mrkF / mrkJ	1..165742	165742
	-	flank	IS/Tn	-	-	91687..91953	266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11033.75 Da
• Isoelectric Point: 10.5388

>T106971 WP_145778088.1 NZ_CP030066:92445-92729 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGTGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T106971 NZ_CP030066:92445-92729 [Klebsiella pneumoniae]
ATGACCTATAAGCTGGCTTTCAACGAATCCGCTCTGAAAGAATGGAAGAAGCTGGGTCATACACTACAGGTACAGTTCAA
AAAGAAACTCAAAGAGCGCCTGGAGAACCCCAGAGTTCCGGCATCACAGCTACATGGACGTAAGGACCAGTATAAAATTA
AGCTACGTGGTACCGGATATCGCCTTGTCTATCGTGTAGAGGACGACATTGTCACAGTCACTGTTATCGGCGTAGGCAAA
CGCGAAAACGACGACATTTATAATGCAACCCTTAATCGCAATTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT106971 WP_004187025.1 NZ_CP030066:92207-92455 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT106971 NZ_CP030066:92207-92455 [Klebsiella pneumoniae]
ATGGCATTTCAGGTTCTGACCACTACAGCAGCTAGCATTACTGAACTTAAACGTGACCCTATGGGTACTTTCCACGCCGG
GGATGGCGCTCCGGTAGCCATTCTTAATCGTAATGAGCCTGCATTCTACTGTGTGCCACCGGCACTCTATGCCCAGTTGA
TGGAGCTTCTGGAAGATGAAGAGCTGGGACGGATGGCTGATGAGCGAAGCGGAGAAAAGGTTATAGAGGTCAATATTGAT
GACCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4