Detailed information of TA system TA106967

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 698290..698882
• Replicon: plasmid unnamed
• Accession: NZ_CP030060
• Organism: Bradyrhizobium sp. CCBAU 53351

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: XH83_RS38265
• Protein ID: WP_128929956.1
• Coordinates: 698290..698478 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: XH83_RS38270
• Protein ID: WP_128929955.1
• Coordinates: 698484..698882 (+)
• Length: 133 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XH83_RS38235	694208..694537	-	330	WP_128930197.1	EAL domain-containing protein	-
XH83_RS38240	694606..694980	+	375	WP_188637391.1	hypothetical protein	-
XH83_RS38245	695157..695630	+	474	WP_128929538.1	transposase	-
XH83_RS38250	695627..695981	+	355	Protein_646	IS66 family insertion sequence element accessory protein TnpB	-
XH83_RS38255	696032..697591	+	1560	WP_128929539.1	IS66 family transposase	-
XH83_RS38260	697624..698214	+	591	WP_128930143.1	UPF0149 family protein	-
XH83_RS38265	698290..698478	+	189	WP_128929956.1	type II toxin-antitoxin system HicA family toxin	Toxin
XH83_RS38270	698484..698882	+	399	WP_128929955.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
XH83_RS38275	699536..700732	-	1197	Protein_651	Fic family protein	-
XH83_RS38280	701210..701788	+	579	WP_128929954.1	helix-turn-helix domain-containing protein	-
XH83_RS38285	701781..703004	+	1224	WP_128929953.1	type II toxin-antitoxin system HipA family toxin	-
XH83_RS38290	703283..703603	-	321	WP_128929952.1	ferredoxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd	1..979290	979290

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7077.32 Da
• Isoelectric Point: 11.2092

>T106967 WP_128929956.1 NZ_CP030060:698290-698478 [Bradyrhizobium sp. CCBAU 53351]
MKSREIISILQGDGWFEVGRKGSHVQFKHPFKKGRVIVPHPERDVPLGTLKSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T106967 NZ_CP030060:698290-698478 [Bradyrhizobium sp. CCBAU 53351]
ATGAAAAGCCGGGAAATTATCTCGATCCTTCAAGGGGATGGATGGTTTGAGGTGGGCCGGAAAGGCAGCCATGTGCAGTT
CAAACATCCATTCAAGAAGGGTCGCGTAATTGTTCCGCACCCCGAACGGGATGTCCCACTCGGGACACTGAAGAGTATTG
AAAAGCAGTCAGGTCTCAAACTGAGGTAA

Antitoxin

Protein

• Length: 133 a.a.
• Molecular weight: 14163.96 Da
• Isoelectric Point: 4.3695

>AT106967 WP_128929955.1 NZ_CP030060:698484-698882 [Bradyrhizobium sp. CCBAU 53351]
MRNYIGLIHKDADSDFGVSFPDFPGVITAGKSLDDARAMAEEALTLHIEGLAEDGEAIPEPSTLEDVMADPDHRTGVAIL
VSVKPEQPKAIRVNVTLPEDVLNQIDKYAEAHGFTRSGLLTQAAKRLIGEAA

Nucleotide

• Length: 399 bp

>AT106967 NZ_CP030060:698484-698882 [Bradyrhizobium sp. CCBAU 53351]
ATGCGTAACTATATTGGTCTTATCCATAAGGATGCCGACAGCGATTTCGGCGTCTCATTTCCAGACTTCCCCGGAGTGAT
CACGGCAGGCAAAAGCCTTGATGACGCTCGTGCTATGGCAGAGGAAGCCCTCACCCTTCATATCGAGGGTCTTGCTGAAG
ATGGAGAAGCCATTCCGGAGCCCTCTACTCTAGAGGATGTTATGGCAGATCCTGATCATCGAACTGGGGTAGCTATTTTG
GTGTCGGTCAAACCCGAACAACCAAAGGCTATACGTGTGAATGTCACCCTTCCTGAGGACGTTCTGAATCAGATCGACAA
ATATGCTGAAGCGCACGGCTTTACGAGATCGGGCCTCCTAACTCAGGCCGCGAAGAGACTCATAGGAGAAGCAGCCTAA