Detailed information of TA system TA106961

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 549561..550153
• Replicon: plasmid unnamed1
• Accession: NZ_CP030054
• Organism: Bradyrhizobium guangzhouense strain CCBAU 51670

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: XH91_RS36575
• Protein ID: WP_128929956.1
• Coordinates: 549965..550153 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: XH91_RS36570
• Protein ID: WP_128929955.1
• Coordinates: 549561..549959 (-)
• Length: 133 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XH91_RS36550	544840..545160	+	321	WP_128929952.1	ferredoxin family protein	-
XH91_RS36555	545439..546662	-	1224	WP_128929953.1	type II toxin-antitoxin system HipA family toxin	-
XH91_RS36560	546655..547233	-	579	WP_128929954.1	helix-turn-helix domain-containing protein	-
XH91_RS36565	547711..548907	+	1197	Protein_492	Fic family protein	-
XH91_RS36570	549561..549959	-	399	WP_128929955.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
XH91_RS36575	549965..550153	-	189	WP_128929956.1	type II toxin-antitoxin system HicA family toxin	Toxin
XH91_RS36580	550229..550819	-	591	WP_128930143.1	UPF0149 family protein	-
XH91_RS36585	550852..552411	-	1560	WP_128929539.1	IS66 family transposase	-
XH91_RS36590	552462..552816	-	355	Protein_497	IS66 family insertion sequence element accessory protein TnpB	-
XH91_RS36595	552813..553286	-	474	WP_128929538.1	transposase	-
XH91_RS36600	553463..553837	-	375	WP_188637391.1	hypothetical protein	-
XH91_RS36605	553906..554235	+	330	WP_128930197.1	EAL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd	1..979291	979291

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7077.32 Da
• Isoelectric Point: 11.2092

>T106961 WP_128929956.1 NZ_CP030054:c550153-549965 [Bradyrhizobium guangzhouense]
MKSREIISILQGDGWFEVGRKGSHVQFKHPFKKGRVIVPHPERDVPLGTLKSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T106961 NZ_CP030054:c550153-549965 [Bradyrhizobium guangzhouense]
ATGAAAAGCCGGGAAATTATCTCGATCCTTCAAGGGGATGGATGGTTTGAGGTGGGCCGGAAAGGCAGCCATGTGCAGTT
CAAACATCCATTCAAGAAGGGTCGCGTAATTGTTCCGCACCCCGAACGGGATGTCCCACTCGGGACACTGAAGAGTATTG
AAAAGCAGTCAGGTCTCAAACTGAGGTAA

Antitoxin

Protein

• Length: 133 a.a.
• Molecular weight: 14163.96 Da
• Isoelectric Point: 4.3695

>AT106961 WP_128929955.1 NZ_CP030054:c549959-549561 [Bradyrhizobium guangzhouense]
MRNYIGLIHKDADSDFGVSFPDFPGVITAGKSLDDARAMAEEALTLHIEGLAEDGEAIPEPSTLEDVMADPDHRTGVAIL
VSVKPEQPKAIRVNVTLPEDVLNQIDKYAEAHGFTRSGLLTQAAKRLIGEAA

Nucleotide

• Length: 399 bp

>AT106961 NZ_CP030054:c549959-549561 [Bradyrhizobium guangzhouense]
ATGCGTAACTATATTGGTCTTATCCATAAGGATGCCGACAGCGATTTCGGCGTCTCATTTCCAGACTTCCCCGGAGTGAT
CACGGCAGGCAAAAGCCTTGATGACGCTCGTGCTATGGCAGAGGAAGCCCTCACCCTTCATATCGAGGGTCTTGCTGAAG
ATGGAGAAGCCATTCCGGAGCCCTCTACTCTAGAGGATGTTATGGCAGATCCTGATCATCGAACTGGGGTAGCTATTTTG
GTGTCGGTCAAACCCGAACAACCAAAGGCTATACGTGTGAATGTCACCCTTCCTGAGGACGTTCTGAATCAGATCGACAA
ATATGCTGAAGCGCACGGCTTTACGAGATCGGGCCTCCTAACTCAGGCCGCGAAGAGACTCATAGGAGAAGCAGCCTAA