Detailed information of TA system TA106954

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 369968..370560
• Replicon: plasmid unnamed
• Accession: NZ_CP030052
• Organism: Bradyrhizobium guangdongense strain CCBAU 51649

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: X265_RS37375
• Protein ID: WP_128929956.1
• Coordinates: 370372..370560 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: X265_RS37370
• Protein ID: WP_128929955.1
• Coordinates: 369968..370366 (-)
• Length: 133 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
X265_RS37350	365247..365567	+	321	WP_128929952.1	ferredoxin family protein	-
X265_RS37355	365846..367069	-	1224	WP_128929953.1	type II toxin-antitoxin system HipA family toxin	-
X265_RS37360	367062..367640	-	579	WP_128929954.1	helix-turn-helix domain-containing protein	-
X265_RS37365	368118..369314	+	1197	Protein_330	Fic family protein	-
X265_RS37370	369968..370366	-	399	WP_128929955.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
X265_RS37375	370372..370560	-	189	WP_128929956.1	type II toxin-antitoxin system HicA family toxin	Toxin
X265_RS37380	370636..371226	-	591	WP_128930143.1	UPF0149 family protein	-
X265_RS37385	371259..372818	-	1560	WP_128929539.1	IS66 family transposase	-
X265_RS37390	372869..373223	-	355	Protein_335	IS66 family insertion sequence element accessory protein TnpB	-
X265_RS37395	373220..373693	-	474	WP_128929538.1	transposase	-
X265_RS37400	373870..374244	-	375	WP_188637391.1	hypothetical protein	-
X265_RS37405	374313..374642	+	330	WP_128930197.1	EAL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd	1..981946	981946

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7077.32 Da
• Isoelectric Point: 11.2092

>T106954 WP_128929956.1 NZ_CP030052:c370560-370372 [Bradyrhizobium guangdongense]
MKSREIISILQGDGWFEVGRKGSHVQFKHPFKKGRVIVPHPERDVPLGTLKSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T106954 NZ_CP030052:c370560-370372 [Bradyrhizobium guangdongense]
ATGAAAAGCCGGGAAATTATCTCGATCCTTCAAGGGGATGGATGGTTTGAGGTGGGCCGGAAAGGCAGCCATGTGCAGTT
CAAACATCCATTCAAGAAGGGTCGCGTAATTGTTCCGCACCCCGAACGGGATGTCCCACTCGGGACACTGAAGAGTATTG
AAAAGCAGTCAGGTCTCAAACTGAGGTAA

Antitoxin

Protein

• Length: 133 a.a.
• Molecular weight: 14163.96 Da
• Isoelectric Point: 4.3695

>AT106954 WP_128929955.1 NZ_CP030052:c370366-369968 [Bradyrhizobium guangdongense]
MRNYIGLIHKDADSDFGVSFPDFPGVITAGKSLDDARAMAEEALTLHIEGLAEDGEAIPEPSTLEDVMADPDHRTGVAIL
VSVKPEQPKAIRVNVTLPEDVLNQIDKYAEAHGFTRSGLLTQAAKRLIGEAA

Nucleotide

• Length: 399 bp

>AT106954 NZ_CP030052:c370366-369968 [Bradyrhizobium guangdongense]
ATGCGTAACTATATTGGTCTTATCCATAAGGATGCCGACAGCGATTTCGGCGTCTCATTTCCAGACTTCCCCGGAGTGAT
CACGGCAGGCAAAAGCCTTGATGACGCTCGTGCTATGGCAGAGGAAGCCCTCACCCTTCATATCGAGGGTCTTGCTGAAG
ATGGAGAAGCCATTCCGGAGCCCTCTACTCTAGAGGATGTTATGGCAGATCCTGATCATCGAACTGGGGTAGCTATTTTG
GTGTCGGTCAAACCCGAACAACCAAAGGCTATACGTGTGAATGTCACCCTTCCTGAGGACGTTCTGAATCAGATCGACAA
ATATGCTGAAGCGCACGGCTTTACGAGATCGGGCCTCCTAACTCAGGCCGCGAAGAGACTCATAGGAGAAGCAGCCTAA