Detailed information of TA system TA106930

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2708117..2708688
• Replicon: chromosome
• Accession: NZ_CP030045
• Organism: Enterococcus faecalis strain C54

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: DOU32_RS13815
• Protein ID: WP_002354774.1
• Coordinates: 2708117..2708458 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: DOU32_RS13820
• Protein ID: WP_002354773.1
• Coordinates: 2708458..2708688 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DOU32_RS13805	2704132..2707746	-	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-
DOU32_RS13815	2708117..2708458	-	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DOU32_RS13820	2708458..2708688	-	231	WP_002354773.1	hypothetical protein	Antitoxin
DOU32_RS13830	2709019..2709234	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
DOU32_RS13835	2709373..2710365	+	993	WP_002358973.1	biotin--[acetyl-CoA-carboxylase] ligase	-
DOU32_RS13840	2710629..2711267	-	639	WP_073438309.1	lytic polysaccharide monooxygenase	-
DOU32_RS13850	2711953..2713569	+	1617	WP_002406969.1	phosphatase PAP2/LCP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T106930 WP_002354774.1 NZ_CP030045:c2708458-2708117 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T106930 NZ_CP030045:c2708458-2708117 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGGCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT106930 WP_002354773.1 NZ_CP030045:c2708688-2708458 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT106930 NZ_CP030045:c2708688-2708458 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GCCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812