Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 70554..71125 Replicon plasmid pC25-2
Accession NZ_CP030044
Organism Enterococcus faecalis strain C25

Toxin (Protein)


Gene name mazF Uniprot ID A0A2Z2CGM4
Locus tag DOU31_RS15045 Protein ID WP_002362432.1
Coordinates 70784..71125 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID R3KHK9
Locus tag DOU31_RS15040 Protein ID WP_002362431.1
Coordinates 70554..70784 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DOU31_RS15005 66959..67210 - 252 WP_002358196.1 hypothetical protein -
DOU31_RS15020 67848..68048 - 201 WP_010817798.1 hypothetical protein -
DOU31_RS15025 68594..69319 - 726 WP_010817797.1 hypothetical protein -
DOU31_RS15035 69730..70350 - 621 WP_025189617.1 recombinase family protein -
DOU31_RS15040 70554..70784 + 231 WP_002362431.1 hypothetical protein Antitoxin
DOU31_RS15045 70784..71125 + 342 WP_002362432.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
DOU31_RS15050 71237..72175 + 939 WP_002362433.1 hypothetical protein -
DOU31_RS15055 72440..73042 + 603 WP_002362434.1 Fic family protein -
DOU31_RS15060 73181..74337 + 1157 WP_100815066.1 IS3 family transposase -
DOU31_RS15065 74443..75726 + 1284 WP_002362438.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - asa1 1..87767 87767


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13244.44 Da        Isoelectric Point: 8.0113

>T106920 WP_002362432.1 NZ_CP030044:70784-71125 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAEKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFAERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

>T106920 NZ_CP030044:70784-71125 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGAAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACATTGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCAACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGACTTTGCAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8510.42 Da        Isoelectric Point: 4.3855

>AT106920 WP_002362431.1 NZ_CP030044:70554-70784 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF

Download         Length: 231 bp

>AT106920 NZ_CP030044:70554-70784 [Enterococcus faecalis]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGACCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z2CGM4

Antitoxin

Source ID Structure
AlphaFold DB R3KHK9

References