106905

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 118517..119088 Replicon chromosome
Accession NZ_CP030042
Organism Enterococcus faecalis strain C25

Toxin (Protein)


Gene name mazF Uniprot ID R3GRA7
Locus tag DOU31_RS00585 Protein ID WP_002360937.1
Coordinates 118747..119088 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S4CGQ1
Locus tag DOU31_RS00580 Protein ID WP_002367500.1
Coordinates 118517..118747 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DOU31_RS00555 113660..115276 - 1617 WP_002372620.1 phosphatase PAP2/LCP family protein -
DOU31_RS00565 115960..116598 + 639 WP_002378863.1 lytic polysaccharide monooxygenase -
DOU31_RS00570 116765..117757 - 993 WP_002398510.1 biotin--[acetyl-CoA-carboxylase] ligase -
DOU31_RS00575 117896..118111 + 216 WP_002354768.1 zinc ribbon domain-containing protein -
DOU31_RS00580 118517..118747 + 231 WP_002367500.1 hypothetical protein Antitoxin
DOU31_RS00585 118747..119088 + 342 WP_002360937.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
DOU31_RS00595 119459..123073 + 3615 WP_002389470.1 DNA-directed RNA polymerase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13165.41 Da        Isoelectric Point: 9.3988

>T106905 WP_002360937.1 NZ_CP030042:118747-119088 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

>T106905 NZ_CP030042:118747-119088 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCATGGGCAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8704.69 Da        Isoelectric Point: 4.3313

>AT106905 WP_002367500.1 NZ_CP030042:118517-118747 [Enterococcus faecalis]
MLTTKSRRQGNSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Download         Length: 231 bp

>AT106905 NZ_CP030042:118517-118747 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAACTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGATGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2A7G9

Antitoxin

Source ID Structure
AlphaFold DB S4CGQ1

References