Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 246032..246668 Replicon chromosome
Accession NZ_CP030028
Organism Bacillus sp. Y1

Toxin (Protein)


Gene name mazF Uniprot ID A0A7V7UTN4
Locus tag DOE78_RS01340 Protein ID WP_019156408.1
Coordinates 246318..246668 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A385NUM8
Locus tag DOE78_RS01335 Protein ID WP_066060032.1
Coordinates 246032..246313 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DOE78_RS01315 242157..242879 - 723 WP_119706358.1 rhomboid family intramembrane serine protease -
DOE78_RS01320 243002..243358 + 357 WP_119706359.1 holo-ACP synthase -
DOE78_RS01325 243531..244547 + 1017 WP_119706360.1 outer membrane lipoprotein carrier protein LolA -
DOE78_RS01330 244672..245832 + 1161 WP_119706361.1 alanine racemase -
DOE78_RS01335 246032..246313 + 282 WP_066060032.1 CopG family ribbon-helix-helix protein Antitoxin
DOE78_RS01340 246318..246668 + 351 WP_019156408.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
DOE78_RS01345 246982..247815 + 834 WP_119706362.1 STAS domain-containing protein -
DOE78_RS01350 247812..248168 + 357 WP_119706363.1 STAS domain-containing protein -
DOE78_RS01355 248173..248574 + 402 WP_119706364.1 anti-sigma regulatory factor -
DOE78_RS01360 248588..249598 + 1011 WP_119706365.1 PP2C family protein-serine/threonine phosphatase -
DOE78_RS01365 249656..249985 + 330 WP_119706366.1 anti-sigma factor antagonist -
DOE78_RS01370 249982..250455 + 474 WP_119706367.1 anti-sigma B factor RsbW -
DOE78_RS01375 250433..251227 + 795 WP_119706368.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13034.08 Da        Isoelectric Point: 4.8998

>T106886 WP_019156408.1 NZ_CP030028:246318-246668 [Bacillus sp. Y1]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIIAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQISLGLIEF

Download         Length: 351 bp

>T106886 NZ_CP030028:246318-246668 [Bacillus sp. Y1]
TTGATTGTCAAACGTGGTGACGTTTATTTTGCAGACCTATCCCCAGTTGTTGGTTCCGAGCAAGGTGGCGTTCGTCCAGT
TCTTGTGATCCAAAACGATATCGGGAATCGGTTCAGTCCCACAGTGATTATTGCAGCCATTACAGCTCAAATTCAGAAAG
CGAAACTGCCTACTCACGTTGAAATTGATGCGAAACGCTATGGTTTCGAACGAGATTCAGTTATCCTGTTGGAACAAATT
CGTACAATTGATAAACAACGTCTGACCGATAAGATCACTCATCTCGATGACGAAATGATGGAGAAAGTGGACGAAGCTTT
ACAAATTAGCCTAGGTCTAATTGAATTCTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10829.36 Da        Isoelectric Point: 7.2028

>AT106886 WP_066060032.1 NZ_CP030028:246032-246313 [Bacillus sp. Y1]
VSESSATTEIMVKLPQHLLTELDGFVKQENVNRSEFIYQATKMYLRERKKRQIRESMRRGYMEMAKINLTIASEAFQAEY
EAEHTVERLVSGG

Download         Length: 282 bp

>AT106886 NZ_CP030028:246032-246313 [Bacillus sp. Y1]
GTGTCTGAGTCCAGCGCAACAACAGAAATTATGGTGAAATTACCGCAACATCTTTTAACCGAATTGGACGGTTTTGTGAA
ACAGGAGAATGTGAACAGAAGCGAATTCATTTATCAAGCTACTAAAATGTACTTGCGTGAGCGGAAAAAGAGACAAATTC
GTGAATCCATGCGAAGAGGCTACATGGAGATGGCGAAGATCAACTTAACAATTGCTTCAGAAGCGTTTCAAGCAGAGTAT
GAGGCAGAACACACAGTTGAACGTCTAGTAAGTGGGGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V7UTN4

Antitoxin

Source ID Structure
AlphaFold DB A0A385NUM8

References