Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) KacT-ataR/DUF1778(antitoxin)
Location 3143165..3143962 Replicon chromosome
Accession NZ_CP030007
Organism Enterobacter hormaechei subsp. xiangfangensis strain Pb204

Toxin (Protein)


Gene name KacT Uniprot ID A0A222RDD1
Locus tag DN066_RS15415 Protein ID WP_032647926.1
Coordinates 3143165..3143686 (-) Length 174 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A331QJY7
Locus tag DN066_RS15420 Protein ID WP_015570876.1
Coordinates 3143693..3143962 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DN066_RS15385 3138763..3139452 + 690 WP_017384771.1 pyrimidine utilization protein B -
DN066_RS15390 3139464..3139850 + 387 WP_015570870.1 pyrimidine utilization protein C -
DN066_RS15395 3139858..3140658 + 801 WP_032647935.1 pyrimidine utilization protein D -
DN066_RS15400 3140668..3141258 + 591 WP_032647932.1 malonic semialdehyde reductase -
DN066_RS15405 3141271..3141762 + 492 WP_032647931.1 pyrimidine utilization flavin reductase protein F -
DN066_RS15410 3141784..3143106 + 1323 WP_032647928.1 pyrimidine utilization transport protein G -
DN066_RS15415 3143165..3143686 - 522 WP_032647926.1 GNAT family N-acetyltransferase Toxin
DN066_RS15420 3143693..3143962 - 270 WP_015570876.1 DUF1778 domain-containing protein Antitoxin
DN066_RS15425 3144036..3144941 - 906 WP_017693666.1 DMT family transporter -
DN066_RS15430 3145058..3145228 - 171 WP_001273664.1 general stress protein -
DN066_RS15435 3145620..3146216 + 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
DN066_RS15440 3146235..3146462 + 228 WP_006809324.1 YccJ family protein -
DN066_RS15445 3146495..3147736 - 1242 WP_045324885.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
DN066_RS15450 3147889..3148053 + 165 WP_017693668.1 helix-turn-helix transcriptional regulator -
DN066_RS15455 3148063..3148422 - 360 WP_032647921.1 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19451.21 Da        Isoelectric Point: 7.4318

>T106851 WP_032647926.1 NZ_CP030007:c3143686-3143165 [Enterobacter hormaechei subsp. xiangfangensis]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLIRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Download         Length: 522 bp

>T106851 NZ_CP030007:c3143686-3143165 [Enterobacter hormaechei subsp. xiangfangensis]
GTGGCAAATCTCACGATCGAAATGTTATCAGAGGGAACCGATTACGATTTCGGCGATTTCGATTGTGGTGAACCCTCGCT
AAATGCCTTCCTTACCGAGCACCTGATACGTCAGCACGGCGGGCGTATTTTACGCGGCTACCTGCTTAAAGAACGCGACC
ATCCGCGTGTCCTGGGCTATTACACACTCTCGGGAAGTTGCTTTGAAAAAGCGATGCTTCCTTCAAAGACTCAGCAGCGC
CGCATCCCATACAGCAACGTCCCCAGCGTGACGCTGGGGCGTCTGGCTGTCCATAAAGAACTCCAGGGAAACGAGTGGGG
AACAACGCTTGTCACACACGCTATGCGCGTTGTTTATCTGGCCTCGCAGGCTGTTGGCGTCCACGGAATTTTTGTGGATG
CGCTTAACGAACGGGCCAAACGATTTTATCTGAAGCTGGGGTTTATCCCGCTGGCAGGTGAAAACAGCAGTTCGCTGTTC
TTCCCCACGCAATCTATAGAACGACTGTTTGAGCAGGCGTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10247.75 Da        Isoelectric Point: 5.8836

>AT106851 WP_015570876.1 NZ_CP030007:c3143962-3143693 [Enterobacter hormaechei subsp. xiangfangensis]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWIAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT106851 NZ_CP030007:c3143962-3143693 [Enterobacter hormaechei subsp. xiangfangensis]
ATGCCAGCACTCAAAAAACAGCGTATCGATCTTCGCCTGTCGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACCAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCCGAAGTCATCGAACAGCATCGGCGTC
TCGTATTAAACGAAGCCTCATGGATTGCCGTTATGGATGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A222RDD1

Antitoxin

Source ID Structure
AlphaFold DB A0A331QJY7

References