Detailed information of TA system TA106828

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 3202101..3202714
• Replicon: chromosome
• Accession: NZ_CP029983
• Organism: Pseudomonas sp. LG1E9

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: DNK50_RS15090
• Protein ID: WP_099584227.1
• Coordinates: 3202463..3202714 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: DNK50_RS15085
• Protein ID: WP_099584226.1
• Coordinates: 3202101..3202466 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DNK50_RS15070	3198422..3199777	+	1356	WP_099584223.1	MATE family efflux transporter	-
DNK50_RS15075	3199780..3200952	+	1173	WP_112194863.1	MFS transporter	-
DNK50_RS15080	3200956..3201954	-	999	WP_112194865.1	Camphene synthase	-
DNK50_RS15085	3202101..3202466	-	366	WP_099584226.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
DNK50_RS15090	3202463..3202714	-	252	WP_099584227.1	type II toxin-antitoxin system HicA family toxin	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9527.06 Da
• Isoelectric Point: 9.7342

>T106828 WP_099584227.1 NZ_CP029983:c3202714-3202463 [Pseudomonas sp. LG1E9]
VSKNEKLLAKLLNEQTIFTWPELVTLLHRLGYTQIEGAGSRVKFDIGDPSAMITLHRPHPGNELKHYIRRQIIEQLRSGE
LIP

Nucleotide

• Length: 252 bp

>T106828 NZ_CP029983:c3202714-3202463 [Pseudomonas sp. LG1E9]
GTGTCAAAAAATGAAAAGCTGCTCGCCAAATTGCTCAACGAGCAAACGATATTCACCTGGCCCGAACTCGTCACACTGTT
ACACCGGCTGGGCTACACACAGATTGAAGGAGCCGGAAGCCGCGTCAAATTCGACATCGGCGATCCCAGTGCAATGATCA
CCTTGCACAGGCCCCACCCCGGCAACGAACTGAAACACTACATTCGGCGCCAGATCATCGAACAATTGCGATCAGGAGAA
CTGATTCCGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13253.11 Da
• Isoelectric Point: 6.3180

>AT106828 WP_099584226.1 NZ_CP029983:c3202466-3202101 [Pseudomonas sp. LG1E9]
VNNQLNYKGYIGSIEASLEDNCLFGKILFIKALVSYEGKTVAELDAAFHEAVDDYLATCQSLGQTPEKPCKGSFNVRVGH
DLHLAAALAAMRKKVTLNDLTRQALNEFLQHHCAETFPAIG

Nucleotide

• Length: 366 bp

>AT106828 NZ_CP029983:c3202466-3202101 [Pseudomonas sp. LG1E9]
GTGAACAACCAACTCAATTACAAAGGCTACATCGGCTCCATCGAAGCCAGCCTCGAAGACAACTGCCTGTTCGGCAAGAT
CCTGTTCATCAAGGCGCTGGTCAGCTATGAAGGTAAAACCGTCGCAGAATTGGATGCGGCATTTCATGAGGCAGTGGACG
ACTACCTGGCTACATGCCAGTCCCTCGGGCAAACACCCGAAAAACCGTGCAAGGGCTCGTTCAATGTACGGGTCGGCCAT
GACCTGCACCTGGCGGCCGCCCTGGCAGCGATGCGCAAAAAAGTGACGCTCAATGATTTGACGCGCCAGGCGCTGAATGA
ATTTCTGCAACATCATTGCGCAGAAACATTCCCCGCGATTGGCTGA