Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1242694..1243348 Replicon chromosome
Accession NZ_CP029981
Organism Escherichia coli strain 99-3165

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag B4O06_RS07645 Protein ID WP_000244781.1
Coordinates 1242941..1243348 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag B4O06_RS07640 Protein ID WP_000354046.1
Coordinates 1242694..1242960 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B4O06_RS07620 1238782..1240215 - 1434 WP_001394742.1 6-phospho-beta-glucosidase BglA -
B4O06_RS07625 1240260..1240571 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
B4O06_RS07630 1240735..1241394 + 660 WP_000250274.1 hemolysin III family protein -
B4O06_RS07635 1241471..1242451 - 981 WP_000886087.1 tRNA-modifying protein YgfZ -
B4O06_RS07640 1242694..1242960 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
B4O06_RS07645 1242941..1243348 + 408 WP_000244781.1 protein YgfX Toxin
B4O06_RS07650 1243388..1243909 - 522 WP_001055874.1 flavodoxin FldB -
B4O06_RS07655 1244021..1244917 + 897 WP_000806650.1 site-specific tyrosine recombinase XerD -
B4O06_RS07660 1244942..1245652 + 711 WP_000715224.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
B4O06_RS07665 1245658..1247391 + 1734 WP_032328035.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T106808 WP_000244781.1 NZ_CP029981:1242941-1243348 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T106808 NZ_CP029981:1242941-1243348 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGAGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT106808 WP_000354046.1 NZ_CP029981:1242694-1242960 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT106808 NZ_CP029981:1242694-1242960 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCATGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References