Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 72376..72901 Replicon plasmid unnamed2
Accession NZ_CP029980
Organism Escherichia coli strain 99-3165

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag B4O06_RS00870 Protein ID WP_001159871.1
Coordinates 72596..72901 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag B4O06_RS00865 Protein ID WP_000813634.1
Coordinates 72376..72594 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B4O06_RS00835 68012..68962 - 951 WP_032328199.1 virulence factor VirK -
B4O06_RS00840 68967..70055 - 1089 WP_064734538.1 putative hexosyltransferase CapU -
B4O06_RS00845 70058..70900 - 843 WP_160371899.1 polysaccharide deacetylase family protein -
B4O06_RS00850 71144..71674 - 531 WP_011387704.1 hypothetical protein -
B4O06_RS00855 71674..71931 - 258 WP_000343092.1 hypothetical protein -
B4O06_RS00865 72376..72594 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
B4O06_RS00870 72596..72901 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
B4O06_RS00875 72902..73615 + 714 WP_112059335.1 site-specific integrase -
B4O06_RS00880 73793..74437 + 645 WP_001144037.1 ParA family protein -
B4O06_RS00885 74524..74832 + 309 WP_000030204.1 molecular chaperone GroEL -
B4O06_RS26770 75042..76272 + 1231 Protein_99 IS66 family transposase -
B4O06_RS00895 76525..77654 - 1130 Protein_100 IS110 family transposase -
B4O06_RS00900 77715..77891 + 177 Protein_101 transposase domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - eltA / eltB / cs3 / etpB 1..117740 117740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T106802 WP_001159871.1 NZ_CP029980:72596-72901 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T106802 NZ_CP029980:72596-72901 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTACTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT106802 WP_000813634.1 NZ_CP029980:72376-72594 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT106802 NZ_CP029980:72376-72594 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References