Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 32867..33392 Replicon plasmid unnamed2
Accession NZ_CP029980
Organism Escherichia coli strain 99-3165

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag B4O06_RS00535 Protein ID WP_001159871.1
Coordinates 32867..33172 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag B4O06_RS00540 Protein ID WP_000813634.1
Coordinates 33174..33392 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B4O06_RS00510 29112..29815 - 704 Protein_34 helix-turn-helix domain-containing protein -
B4O06_RS00515 30063..30742 + 680 Protein_35 EAL domain-containing protein -
B4O06_RS00525 31182..31823 - 642 WP_001705742.1 recombinase family protein -
B4O06_RS00530 32153..32866 - 714 WP_000016957.1 site-specific integrase -
B4O06_RS00535 32867..33172 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
B4O06_RS00540 33174..33392 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
B4O06_RS00550 33837..34094 + 258 WP_000343092.1 hypothetical protein -
B4O06_RS00555 34094..34624 + 531 WP_011387704.1 hypothetical protein -
B4O06_RS00560 34868..35710 + 843 WP_160371899.1 polysaccharide deacetylase family protein -
B4O06_RS00565 35713..36801 + 1089 WP_064734538.1 putative hexosyltransferase CapU -
B4O06_RS00570 36806..37756 + 951 WP_032328199.1 virulence factor VirK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - eltA / eltB / cs3 / etpB 1..117740 117740
- flank IS/Tn - - 31182..31823 641


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T106801 WP_001159871.1 NZ_CP029980:c33172-32867 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T106801 NZ_CP029980:c33172-32867 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTACTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT106801 WP_000813634.1 NZ_CP029980:c33392-33174 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT106801 NZ_CP029980:c33392-33174 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References