106800

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 2281..2774 Replicon plasmid unnamed1
Accession NZ_CP029979
Organism Escherichia coli strain 99-3165

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag B4O06_RS00035 Protein ID WP_001230709.1
Coordinates 2520..2774 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag B4O06_RS00030 Protein ID WP_000151456.1
Coordinates 2281..2523 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B4O06_RS00010 164..397 - 234 WP_000093745.1 hypothetical protein -
B4O06_RS00015 825..1112 - 288 WP_000606467.1 hypothetical protein -
B4O06_RS26730 1203..1340 - 138 WP_001330659.1 hypothetical protein -
B4O06_RS00020 1417..1710 - 294 WP_000673431.1 hypothetical protein -
B4O06_RS26735 2027..2194 + 168 WP_001336599.1 hypothetical protein -
B4O06_RS00030 2281..2523 - 243 WP_000151456.1 hypothetical protein Antitoxin
B4O06_RS00035 2520..2774 - 255 WP_001230709.1 hypothetical protein Toxin
B4O06_RS00040 2771..3313 - 543 WP_001083904.1 hypothetical protein -
B4O06_RS00045 3417..3932 - 516 WP_001025393.1 J domain-containing protein -
B4O06_RS00055 4766..5116 - 351 WP_000127482.1 addiction module antitoxin -
B4O06_RS00060 5103..5447 - 345 WP_001146936.1 type II toxin-antitoxin system RelE/ParE family toxin -
B4O06_RS00070 5813..7021 + 1209 WP_112059332.1 IS4 family transposase -
B4O06_RS00075 7037..7156 - 120 Protein_12 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(B) - 1..36564 36564


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T106800 WP_001230709.1 NZ_CP029979:c2774-2520 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T106800 NZ_CP029979:c2774-2520 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT106800 WP_000151456.1 NZ_CP029979:c2523-2281 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT106800 NZ_CP029979:c2523-2281 [Escherichia coli]
ATGACTATCGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References