Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 18280..18805 Replicon plasmid p51008369SK1_E
Accession NZ_CP029978
Organism Escherichia coli strain 51008369SK1

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag DNQ41_RS26900 Protein ID WP_001159871.1
Coordinates 18500..18805 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag DNQ41_RS26895 Protein ID WP_000813630.1
Coordinates 18280..18498 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DNQ41_RS26860 13490..14176 + 687 Protein_11 IS1 family transposase -
DNQ41_RS26870 14430..15452 - 1023 WP_000361402.1 helicase UvrD -
DNQ41_RS26875 15437..17002 - 1566 WP_001128474.1 AAA family ATPase -
DNQ41_RS26880 17077..17493 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
DNQ41_RS26885 17490..17720 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
DNQ41_RS26895 18280..18498 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
DNQ41_RS26900 18500..18805 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
DNQ41_RS26905 18806..19612 + 807 WP_000016970.1 site-specific integrase -
DNQ41_RS26910 20334..21089 + 756 WP_000852146.1 replication initiation protein RepE -
DNQ41_RS26915 21677..22843 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / aac(3)-IIa / mph(A) iucA / iucB / iucC / iucD / iutA 1..99465 99465


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T106795 WP_001159871.1 NZ_CP029978:18500-18805 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T106795 NZ_CP029978:18500-18805 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT106795 WP_000813630.1 NZ_CP029978:18280-18498 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT106795 NZ_CP029978:18280-18498 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References