Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 17077..17720 Replicon plasmid p51008369SK1_E
Accession NZ_CP029978
Organism Escherichia coli strain 51008369SK1

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag DNQ41_RS26880 Protein ID WP_001034044.1
Coordinates 17077..17493 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag DNQ41_RS26885 Protein ID WP_001261286.1
Coordinates 17490..17720 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DNQ41_RS26860 13490..14176 + 687 Protein_11 IS1 family transposase -
DNQ41_RS26870 14430..15452 - 1023 WP_000361402.1 helicase UvrD -
DNQ41_RS26875 15437..17002 - 1566 WP_001128474.1 AAA family ATPase -
DNQ41_RS26880 17077..17493 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
DNQ41_RS26885 17490..17720 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
DNQ41_RS26895 18280..18498 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
DNQ41_RS26900 18500..18805 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
DNQ41_RS26905 18806..19612 + 807 WP_000016970.1 site-specific integrase -
DNQ41_RS26910 20334..21089 + 756 WP_000852146.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / aac(3)-IIa / mph(A) iucA / iucB / iucC / iucD / iutA 1..99465 99465


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T106794 WP_001034044.1 NZ_CP029978:c17493-17077 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T106794 NZ_CP029978:c17493-17077 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT106794 WP_001261286.1 NZ_CP029978:c17720-17490 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT106794 NZ_CP029978:c17720-17490 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References