Detailed information of TA system TA106778

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4006566..4007398
• Replicon: chromosome
• Accession: NZ_CP029973
• Organism: Escherichia coli strain 51008369SK1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: DNQ41_RS20435
• Protein ID: WP_000854765.1
• Coordinates: 4007024..4007398 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: DNQ41_RS20430
• Protein ID: WP_001295723.1
• Coordinates: 4006566..4006934 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DNQ41_RS20395	4002230..4002910	+	681	WP_001282921.1	WYL domain-containing protein	-
DNQ41_RS20400	4003058..4003735	+	678	WP_001097302.1	hypothetical protein	-
DNQ41_RS20405	4003741..4003974	+	234	WP_001278293.1	DUF905 family protein	-
DNQ41_RS20410	4004064..4004882	+	819	WP_001175165.1	DUF945 domain-containing protein	-
DNQ41_RS20415	4005148..4005627	+	480	WP_057109541.1	antirestriction protein	-
DNQ41_RS20420	4005643..4006119	+	477	WP_001186774.1	RadC family protein	-
DNQ41_RS20425	4006182..4006403	+	222	WP_000692341.1	DUF987 domain-containing protein	-
DNQ41_RS20430	4006566..4006934	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DNQ41_RS20435	4007024..4007398	+	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
DNQ41_RS20440	4007398..4007601	+	204	WP_001398797.1	hypothetical protein	-
DNQ41_RS20445	4007626..4007820	+	195	WP_024181941.1	DUF957 domain-containing protein	-
DNQ41_RS20450	4007905..4008747	+	843	WP_022645115.1	DUF4942 domain-containing protein	-
DNQ41_RS20455	4009028..4009564	-	537	WP_000942785.1	GspM family type II secretion system protein YghD	-
DNQ41_RS20460	4009566..4010744	-	1179	WP_000094991.1	type II secretion system protein GspL	-
DNQ41_RS20465	4010741..4011718	-	978	WP_000633209.1	type II secretion system minor pseudopilin GspK	-
DNQ41_RS20470	4011721..4012320	-	600	WP_001255034.1	type II secretion system minor pseudopilin GspJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T106778 WP_000854765.1 NZ_CP029973:4007024-4007398 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T106778 NZ_CP029973:4007024-4007398 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT106778 WP_001295723.1 NZ_CP029973:4006566-4006934 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT106778 NZ_CP029973:4006566-4006934 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52