Detailed information of TA system TA106757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09907-MazE
• Location: 63035..63636
• Replicon: plasmid pDH5
• Accession: NZ_CP029972
• Organism: Lentilactobacillus kefiri strain DH5

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: DNL43_RS11980
• Protein ID: WP_095347959.1
• Coordinates: 63035..63379 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A806J926
• Locus tag: DNL43_RS11985
• Protein ID: WP_001748109.1
• Coordinates: 63373..63636 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DNL43_RS11950	58057..58977	+	921	WP_003646535.1	prenyltransferase	-
DNL43_RS11955	59090..59452	+	363	WP_135294559.1	transposase	-
DNL43_RS11960	59411..59757	-	347	Protein_71	transposase	-
DNL43_RS11965	60067..61014	+	948	WP_095347957.1	magnesium transporter CorA family protein	-
DNL43_RS11970	61118..61633	-	516	WP_054769888.1	dihydrofolate reductase family protein	-
DNL43_RS11975	62126..62890	+	765	WP_095347958.1	helix-turn-helix domain-containing protein	-
DNL43_RS11980	63035..63379	-	345	WP_095347959.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DNL43_RS11985	63373..63636	-	264	WP_001748109.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DNL43_RS11990	63954..64922	+	969	WP_156645097.1	IS30 family transposase	-
DNL43_RS11995	64956..65312	-	357	WP_002834562.1	type II toxin-antitoxin system YafQ family toxin	-
DNL43_RS12000	65312..65590	-	279	WP_002834561.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
DNL43_RS12140	66644..67153	+	510	WP_179286863.1	Mobilization protein MobB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..67759	67759

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-101)

Antitoxin

• Antitoxin PemI-like (8-85)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13033.78 Da
• Isoelectric Point: 5.1121

>T106757 WP_095347959.1 NZ_CP029972:c63379-63035 [Lentilactobacillus kefiri]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTERNFPMYHRLTSSQTVHGKVLLDQTIALDLR
ARHVTDEAIVDHVSREELEEIIFLYKLLFSIDDE

Nucleotide

• Length: 345 bp

>T106757 NZ_CP029972:c63379-63035 [Lentilactobacillus kefiri]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGGCCAGCCAT
TGCTCTAAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCGAGCGTAATTTTC
CTATGTATCATCGGCTAACTAGTAGTCAAACAGTACATGGGAAAGTATTATTAGATCAAACAATAGCCTTAGATTTGCGG
GCAAGACATGTCACTGATGAAGCCATTGTTGATCATGTATCGCGTGAAGAATTAGAAGAAATAATCTTTTTATACAAATT
GTTGTTCAGTATTGATGACGAATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10141.47 Da
• Isoelectric Point: 5.7117

>AT106757 WP_001748109.1 NZ_CP029972:c63636-63373 [Lentilactobacillus kefiri]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Nucleotide

• Length: 264 bp

>AT106757 NZ_CP029972:c63636-63373 [Lentilactobacillus kefiri]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCTAAGGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAGGATTGGCAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A806J926