Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2067385..2067974 Replicon chromosome
Accession NZ_CP029829
Organism Azospirillum ramasamyi strain M2T2B2

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag DM194_RS09640 Protein ID WP_111067119.1
Coordinates 2067627..2067974 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag DM194_RS09635 Protein ID WP_111067118.1
Coordinates 2067385..2067630 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DM194_RS09615 2062830..2063027 + 198 WP_111067115.1 DUF2783 domain-containing protein -
DM194_RS09620 2063145..2064527 + 1383 WP_111067116.1 alpha/beta hydrolase -
DM194_RS09625 2064605..2065132 - 528 WP_111067886.1 translation initiation factor IF-3 -
DM194_RS09630 2065217..2067181 - 1965 WP_111067117.1 threonine--tRNA ligase -
DM194_RS09635 2067385..2067630 + 246 WP_111067118.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
DM194_RS09640 2067627..2067974 + 348 WP_111067119.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
DM194_RS09645 2067985..2069202 - 1218 WP_111067120.1 EAL domain-containing protein -
DM194_RS09650 2069239..2070312 - 1074 WP_111067121.1 quinone-dependent dihydroorotate dehydrogenase -
DM194_RS09655 2070309..2070683 - 375 WP_111067122.1 DUF952 domain-containing protein -
DM194_RS09660 2071055..2071273 + 219 WP_111067123.1 hypothetical protein -
DM194_RS09665 2071321..2071641 - 321 WP_111067124.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-111)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12417.35 Da        Isoelectric Point: 9.6230

>T106744 WP_111067119.1 NZ_CP029829:2067627-2067974 [Azospirillum ramasamyi]
MTGDEWEPDAGDLIWTDFDPRTGREQGGRRPALIVSPRAFWQATRFAIVCPITTRVRPFPSSAVLPPGPPISGEILTSHV
RSIDTMARPLLFSGARAPSAILAEVRGKLAALIGL

Download         Length: 348 bp

>T106744 NZ_CP029829:2067627-2067974 [Azospirillum ramasamyi]
ATGACCGGAGACGAATGGGAGCCGGATGCCGGCGATCTCATCTGGACCGACTTCGATCCCCGAACGGGGCGTGAACAGGG
CGGACGCAGACCGGCACTGATCGTGTCACCGCGTGCATTCTGGCAAGCGACGCGCTTTGCAATCGTATGCCCCATCACCA
CGCGCGTCCGCCCATTTCCCAGCAGTGCCGTGCTGCCGCCAGGCCCGCCGATTTCCGGCGAAATCCTCACCAGCCATGTT
CGCAGCATCGATACCATGGCCCGGCCACTGCTGTTCTCCGGCGCACGCGCCCCATCGGCCATTCTGGCGGAAGTGCGTGG
AAAGCTTGCGGCACTGATCGGACTTTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9159.39 Da        Isoelectric Point: 4.6286

>AT106744 WP_111067118.1 NZ_CP029829:2067385-2067630 [Azospirillum ramasamyi]
MQVQLARWGNSLGIRIPKDIANQVGLRDGARVDIHAEGDRIIITTERPRYSLDELMKGVTPEAMREAFDWGPDVGREIVE
E

Download         Length: 246 bp

>AT106744 NZ_CP029829:2067385-2067630 [Azospirillum ramasamyi]
ATGCAGGTCCAACTCGCCCGGTGGGGCAACAGCCTGGGCATCCGCATCCCCAAGGACATTGCCAATCAAGTGGGGCTGCG
CGACGGCGCAAGGGTGGACATTCACGCAGAAGGCGATCGGATCATCATTACCACGGAGCGCCCCCGCTACAGCCTGGACG
AACTTATGAAAGGCGTCACGCCTGAGGCGATGCGGGAGGCGTTCGACTGGGGTCCCGATGTCGGTCGCGAGATCGTCGAA
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References