Detailed information of TA system TA106724

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 149506..150097
• Replicon: plasmid unnamed2
• Accession: NZ_CP029776
• Organism: Deinococcus actinosclerus strain Deinococcus actinosclerus SJTR

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: DM785_RS17130
• Protein ID: WP_110832813.1
• Coordinates: 149744..150097 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: DM785_RS17125
• Protein ID: WP_110832811.1
• Coordinates: 149506..149757 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DM785_RS17085	144789..145186	+	398	Protein_100	MerR family DNA-binding protein	-
DM785_RS17090	145164..145905	+	742	Protein_101	MBL fold metallo-hydrolase	-
DM785_RS17095	145965..146093	-	129	WP_110832803.1	SDR family oxidoreductase	-
DM785_RS17100	146087..146392	-	306	WP_162621485.1	SDR family oxidoreductase	-
DM785_RS19735	146635..147504	+	870	WP_204354206.1	type I restriction endonuclease subunit R	-
DM785_RS19740	147434..147943	+	510	WP_204354210.1	hypothetical protein	-
DM785_RS17115	147962..148171	+	210	WP_162621486.1	transposase	-
DM785_RS17120	148427..149377	-	951	WP_110832809.1	tyrosine-type recombinase/integrase	-
DM785_RS17125	149506..149757	+	252	WP_110832811.1	antitoxin	Antitoxin
DM785_RS17130	149744..150097	+	354	WP_110832813.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DM785_RS17135	150560..151651	+	1092	WP_110827773.1	transposase	-
DM785_RS17140	151631..152020	-	390	WP_162621487.1	hypothetical protein	-
DM785_RS17145	152090..152806	-	717	WP_162621488.1	hypothetical protein	-
DM785_RS17150	153047..153382	+	336	WP_110832820.1	hypothetical protein	-
DM785_RS17155	153452..153844	-	393	WP_162621489.1	hypothetical protein	-
DM785_RS17160	154071..154277	+	207	WP_110832824.1	hypothetical protein	-
DM785_RS17165	154400..155080	+	681	WP_110832826.1	SMI1/KNR4 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..187951	187951
	-	flank	IS/Tn	-	-	143601..144692	1091
	-	flank	IS/Tn	-	-	150560..151651	1091

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 12851.79 Da
• Isoelectric Point: 9.8828

>T106724 WP_110832813.1 NZ_CP029776:149744-150097 [Deinococcus actinosclerus]
MRRGDLYLADLDPARPSEADKRRPVVIVSSDAMNRTVDRLGAGAVTVVPVTSNVTRIYDFQVLLPAERTGLTQDSKAQAE
QIRTISFSRLSAAPIGVVPPELMRRLDDALRLHLALT

Nucleotide

• Length: 354 bp

>T106724 NZ_CP029776:149744-150097 [Deinococcus actinosclerus]
ATGCGCCGAGGTGATCTCTACCTCGCCGATCTGGACCCGGCCCGACCCAGCGAAGCCGACAAGCGGCGGCCCGTCGTCAT
CGTCAGCAGCGACGCCATGAACCGCACTGTTGATCGTCTGGGCGCCGGGGCCGTCACCGTGGTGCCCGTCACCTCGAATG
TCACCCGGATCTACGATTTCCAGGTGCTGCTCCCAGCAGAGCGAACGGGCCTGACTCAGGACAGCAAAGCGCAGGCGGAG
CAGATCCGCACCATCAGTTTCAGTCGCCTGAGCGCTGCCCCCATCGGTGTGGTGCCACCAGAGCTGATGCGACGACTCGA
TGACGCGCTGCGGCTGCACCTCGCCCTGACGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9238.19 Da
• Isoelectric Point: 4.4316

>AT106724 WP_110832811.1 NZ_CP029776:149506-149757 [Deinococcus actinosclerus]
MTVQKTSVSIDAQLLAFAEHYQVTHHLRSRSEVFAEALTLLRERELETQYAAALSEWHASDDEDLWDAVAGDLLTSGGPN
APR

Nucleotide

• Length: 252 bp

>AT106724 NZ_CP029776:149506-149757 [Deinococcus actinosclerus]
ATGACTGTTCAGAAAACCAGTGTGAGCATTGATGCCCAGCTGTTGGCCTTCGCTGAGCACTACCAAGTCACCCACCACCT
CCGCTCCCGTTCCGAGGTGTTCGCTGAGGCCCTGACACTCCTGCGGGAACGAGAACTCGAAACGCAATACGCTGCGGCCC
TCAGCGAATGGCACGCCTCTGACGACGAGGACCTGTGGGATGCGGTCGCCGGTGACCTCCTGACCAGCGGAGGTCCGAAT
GCGCCGAGGTGA