Detailed information of TA system TA106723

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 961860..962419
• Replicon: chromosome
• Accession: NZ_CP029756
• Organism: Brucella melitensis strain CIIMS-NV-1

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A7U8PD14
• Locus tag: DMS17_RS05045
• Protein ID: WP_002966807.1
• Coordinates: 962174..962419 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: C0RIV1
• Locus tag: DMS17_RS05040
• Protein ID: WP_004683740.1
• Coordinates: 961860..962174 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DMS17_RS04990	957565..957768	-	204	WP_004683737.1	hypothetical protein	-
DMS17_RS04995	957771..957986	-	216	WP_005968947.1	hypothetical protein	-
DMS17_RS05000	957983..958186	-	204	WP_002964088.1	hypothetical protein	-
DMS17_RS05005	958236..958940	+	705	WP_005968944.1	hypothetical protein	-
DMS17_RS05010	958937..959167	+	231	WP_002964091.1	hypothetical protein	-
DMS17_RS05015	959164..959439	+	276	WP_002964092.1	hypothetical protein	-
DMS17_RS05020	959462..959938	+	477	WP_002966806.1	hypothetical protein	-
DMS17_RS05025	960284..960994	+	711	WP_004683739.1	porin family protein	-
DMS17_RS05030	961342..961512	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
DMS17_RS05040	961860..962174	-	315	WP_004683740.1	BrnA antitoxin family protein	Antitoxin
DMS17_RS05045	962174..962419	-	246	WP_002966807.1	BrnT family toxin	Toxin
DMS17_RS05050	962552..963190	+	639	Protein_923	tRNA-guanine transglycosylase	-
DMS17_RS05055	963260..964018	+	759	Protein_924	IS5 family transposase	-
DMS17_RS05060	964019..964711	+	693	WP_110506818.1	hypothetical protein	-
DMS17_RS05065	965123..966574	+	1452	WP_006135932.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	955793..969890	14097
	-	flank	IS/Tn	-	-	963260..963628	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9162.82 Da
• Isoelectric Point: 10.3158

>T106723 WP_002966807.1 NZ_CP029756:c962419-962174 [Brucella melitensis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Nucleotide

• Length: 246 bp

>T106723 NZ_CP029756:c962419-962174 [Brucella melitensis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11653.48 Da
• Isoelectric Point: 10.5824

>AT106723 WP_004683740.1 NZ_CP029756:c962174-961860 [Brucella melitensis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT106723 NZ_CP029756:c962174-961860 [Brucella melitensis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U8PD14

Antitoxin

• AlphaFold DB: C0RIV1