Detailed information of TA system TA106718

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2189825..2190354
• Replicon: chromosome
• Accession: NZ_CP029751
• Organism: Staphylococcus aureus strain Smith

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CFC57_RS11320
• Protein ID: WP_000621175.1
• Coordinates: 2189825..2190187 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: CFC57_RS11325
• Protein ID: WP_000948331.1
• Coordinates: 2190184..2190354 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFC57_RS11300	2186804..2187574	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
CFC57_RS11305	2187549..2188028	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
CFC57_RS11310	2188030..2188356	-	327	WP_001052491.1	anti-sigma factor antagonist	-
CFC57_RS11315	2188475..2189476	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
CFC57_RS11320	2189825..2190187	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CFC57_RS11325	2190184..2190354	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
CFC57_RS11330	2190439..2191587	-	1149	WP_001281161.1	alanine racemase	-
CFC57_RS11335	2191653..2192012	-	360	WP_000581197.1	holo-ACP synthase	-
CFC57_RS11340	2192016..2192507	-	492	WP_001205921.1	PH domain-containing protein	-
CFC57_RS11345	2192494..2194077	-	1584	WP_001294644.1	PH domain-containing protein	-
CFC57_RS11350	2194070..2194549	-	480	WP_000118170.1	hypothetical protein	-
CFC57_RS11355	2194758..2195318	-	561	WP_001092418.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T106718 WP_000621175.1 NZ_CP029751:c2190187-2189825 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T106718 NZ_CP029751:c2190187-2189825 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGATTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT106718 WP_000948331.1 NZ_CP029751:c2190354-2190184 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT106718 NZ_CP029751:c2190354-2190184 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1