Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4738751..4739367 Replicon chromosome
Accession NZ_CP029747
Organism Escherichia coli strain 2016C-3878

Toxin (Protein)


Gene name vapC Uniprot ID A0A176ZIX8
Locus tag BEN53_RS24125 Protein ID WP_001129490.1
Coordinates 4738993..4739367 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1M0CY48
Locus tag BEN53_RS24120 Protein ID WP_001275523.1
Coordinates 4738751..4738993 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BEN53_RS24090 4733986..4734423 + 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
BEN53_RS24095 4734468..4735409 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
BEN53_RS24100 4735825..4737639 + 1815 WP_000226324.1 hypothetical protein -
BEN53_RS24115 4738313..4738531 + 219 WP_001251290.1 ribbon-helix-helix domain-containing protein -
BEN53_RS24120 4738751..4738993 + 243 WP_001275523.1 ribbon-helix-helix domain-containing protein Antitoxin
BEN53_RS24125 4738993..4739367 + 375 WP_001129490.1 type II toxin-antitoxin system VapC family toxin Toxin
BEN53_RS24130 4739404..4740333 - 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
BEN53_RS24135 4740330..4740965 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
BEN53_RS24140 4740962..4741864 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-115)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-48)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13960.19 Da        Isoelectric Point: 7.0126

>T106694 WP_001129490.1 NZ_CP029747:4738993-4739367 [Escherichia coli]
MAKRTALFDTNILIDLFSGRIEAKQALEAWPLQNAISLVTWMEVLVGAKKYDQEHRTRIAMSAFNVIGISQDIAERSVAL
RQEYRMKLPDAIILATAQIYGFELVTRNTRDFAGIAGVVTPYEL

Download         Length: 375 bp

>T106694 NZ_CP029747:4738993-4739367 [Escherichia coli]
ATGGCAAAAAGAACTGCGCTTTTTGATACCAATATCCTTATCGATTTGTTCAGCGGACGAATTGAGGCAAAACAGGCTCT
GGAGGCATGGCCCCTACAGAATGCGATTAGTCTGGTCACATGGATGGAGGTGCTGGTTGGCGCAAAAAAATATGATCAAG
AACATCGCACACGGATTGCAATGAGTGCGTTTAACGTTATTGGGATTTCGCAGGATATCGCGGAACGAAGCGTTGCTTTA
AGGCAGGAGTATAGAATGAAACTTCCAGATGCCATTATTCTGGCAACAGCACAGATTTATGGCTTTGAACTGGTAACGCG
AAATACGAGAGATTTTGCGGGAATAGCAGGTGTCGTCACACCCTATGAGCTGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9290.54 Da        Isoelectric Point: 4.3774

>AT106694 WP_001275523.1 NZ_CP029747:4738751-4738993 [Escherichia coli]
MSAMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVDQYLENQSKTTISSAFGIWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT106694 NZ_CP029747:4738751-4738993 [Escherichia coli]
ATGAGCGCTATGGCAGGTATGGATATGGGCAGAATTTTACTCGATTTATCCGATGAGGTGATTAAGCGACTTGATGATCT
CAAAGTGCAGCGCAATCTTCCTCGCGCAGAGCTATTACGAGAAGCTGTAGATCAATATCTGGAAAATCAATCGAAAACGA
CAATATCCAGTGCTTTTGGTATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A176ZIX8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0CY48

References