Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 4289405..4290017 Replicon chromosome
Accession NZ_CP029747
Organism Escherichia coli strain 2016C-3878

Toxin (Protein)


Gene name hicA Uniprot ID U9YXE2
Locus tag BEN53_RS21875 Protein ID WP_000833473.1
Coordinates 4289832..4290017 (-) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1M2IVK0
Locus tag BEN53_RS21870 Protein ID WP_000499742.1
Coordinates 4289405..4289815 (-) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BEN53_RS21855 4284862..4285677 - 816 WP_000891820.1 AraC family transcriptional regulator -
BEN53_RS21860 4285903..4287303 + 1401 WP_000204814.1 MFS transporter -
BEN53_RS21865 4287314..4289293 + 1980 WP_001026863.1 glycoside hydrolase family 127 protein -
BEN53_RS21870 4289405..4289815 - 411 WP_000499742.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
BEN53_RS21875 4289832..4290017 - 186 WP_000833473.1 type II toxin-antitoxin system HicA family toxin Toxin
BEN53_RS21885 4290490..4291569 + 1080 WP_000061475.1 type I restriction enzyme HsdR N-terminal domain-containing protein -
BEN53_RS21890 4291610..4293148 - 1539 WP_000183978.1 aldehyde dehydrogenase AldB -
BEN53_RS21895 4293256..4294551 - 1296 WP_000985745.1 Fic family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6800.89 Da        Isoelectric Point: 11.7053

>T106692 WP_000833473.1 NZ_CP029747:c4290017-4289832 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Download         Length: 186 bp

>T106692 NZ_CP029747:c4290017-4289832 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15242.13 Da        Isoelectric Point: 4.4482

>AT106692 WP_000499742.1 NZ_CP029747:c4289815-4289405 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMDEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Download         Length: 411 bp

>AT106692 NZ_CP029747:c4289815-4289405 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGG
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A1M2IVK0

References