Detailed information of TA system TA106675

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1313478..1314183
• Replicon: chromosome
• Accession: NZ_CP029747
• Organism: Escherichia coli strain 2016C-3878

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1M2J6W7
• Locus tag: BEN53_RS06790
• Protein ID: WP_000539524.1
• Coordinates: 1313797..1314183 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: BEN53_RS06785
• Protein ID: WP_001280945.1
• Coordinates: 1313478..1313807 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEN53_RS06770	1308647..1309558	+	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-
BEN53_RS06775	1309735..1312083	+	2349	WP_000950331.1	EAL domain-containing protein	-
BEN53_RS06780	1312091..1313408	+	1318	Protein_1240	diguanylate cyclase	-
BEN53_RS06785	1313478..1313807	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
BEN53_RS06790	1313797..1314183	-	387	WP_000539524.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BEN53_RS06795	1314409..1315734	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
BEN53_RS06800	1315947..1316330	+	384	WP_000497137.1	biofilm formation regulator BssR	-
BEN53_RS06805	1316441..1317556	+	1116	WP_000555056.1	aldose sugar dehydrogenase YliI	-
BEN53_RS06810	1317553..1318179	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14321.46 Da
• Isoelectric Point: 9.9521

>T106675 WP_000539524.1 NZ_CP029747:c1314183-1313797 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSRSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T106675 NZ_CP029747:c1314183-1313797 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAAGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAGGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT106675 WP_001280945.1 NZ_CP029747:c1313807-1313478 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT106675 NZ_CP029747:c1313807-1313478 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1M2J6W7