Detailed information of TA system TA106672

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 852058..852895
• Replicon: chromosome
• Accession: NZ_CP029747
• Organism: Escherichia coli strain 2016C-3878

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A1M0D5U3
• Locus tag: BEN53_RS04330
• Protein ID: WP_000227782.1
• Coordinates: 852058..852600 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: B1LJI1
• Locus tag: BEN53_RS04335
• Protein ID: WP_001353405.1
• Coordinates: 852584..852895 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEN53_RS04305	847136..847726	-	591	WP_001276320.1	protein deglycase YajL	-
BEN53_RS04310	847689..848600	-	912	WP_000705841.1	2-dehydropantoate 2-reductase	-
BEN53_RS04315	848768..849259	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
BEN53_RS04320	849387..850751	-	1365	WP_001000978.1	MFS transporter	-
BEN53_RS04325	851158..852002	+	845	Protein_795	sel1 repeat family protein	-
BEN53_RS04330	852058..852600	-	543	WP_000227782.1	GNAT family N-acetyltransferase	Toxin
BEN53_RS04335	852584..852895	-	312	WP_001353405.1	DUF1778 domain-containing protein	Antitoxin
BEN53_RS04340	853080..853970	-	891	WP_000971327.1	heme o synthase	-
BEN53_RS04345	853982..854311	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
BEN53_RS04350	854311..854925	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
BEN53_RS04355	854915..856906	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
BEN53_RS04360	856928..857875	-	948	WP_001239437.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19957.31 Da
• Isoelectric Point: 9.1763

>T106672 WP_000227782.1 NZ_CP029747:c852600-852058 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDIIYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCNFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T106672 NZ_CP029747:c852600-852058 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTATTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCTGCTAAAGCTCTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAGATTGGTGTTGTCAGATTAGTCATGTTAGGGGTAGCGCG
GAAATATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTAATTTTTTTGAACATGTAAAAATAATTCACCGGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATACTCGCCTCGGCTTTGTTCAGCTTTCAGCG
AGGCCCAATGTTTTCGGTGCTGTACCGATGTTTTTAGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11370.09 Da
• Isoelectric Point: 10.4825

>AT106672 WP_001353405.1 NZ_CP029747:c852895-852584 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT106672 NZ_CP029747:c852895-852584 [Escherichia coli]
ATGCCTGGAAAAACAGTCGCTCTACCCGATATCGATAAAACGCTGAAAAATGCGCGGGTTGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCAGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A1M0D5U3

Antitoxin

• AlphaFold DB: A0A829G7G3