Detailed information of TA system TA106668

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 68069..68904
• Replicon: chromosome
• Accession: NZ_CP029747
• Organism: Escherichia coli strain 2016C-3878

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1EZ92
• Locus tag: BEN53_RS00345
• Protein ID: WP_000854726.1
• Coordinates: 68069..68446 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: BEN53_RS00350
• Protein ID: WP_064226358.1
• Coordinates: 68536..68904 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEN53_RS00320	64161..65699	-	1539	WP_061092756.1	lysine decarboxylation/transport transcriptional activator CadC	-
BEN53_RS00330	66448..67290	-	843	WP_077737641.1	DUF4942 domain-containing protein	-
BEN53_RS00335	67375..67572	-	198	WP_000839269.1	DUF957 domain-containing protein	-
BEN53_RS00340	67584..68072	-	489	WP_063100845.1	hypothetical protein	-
BEN53_RS00345	68069..68446	-	378	WP_000854726.1	TA system toxin CbtA family protein	Toxin
BEN53_RS00350	68536..68904	-	369	WP_064226358.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BEN53_RS00355	68983..69204	-	222	WP_000692312.1	DUF987 domain-containing protein	-
BEN53_RS00360	69273..69749	-	477	WP_064226357.1	RadC family protein	-
BEN53_RS00365	69764..70249	-	486	WP_110826407.1	antirestriction protein	-
BEN53_RS00370	70340..71158	-	819	WP_077737897.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14164.10 Da
• Isoelectric Point: 7.8045

>T106668 WP_000854726.1 NZ_CP029747:c68446-68069 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T106668 NZ_CP029747:c68446-68069 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13652.36 Da
• Isoelectric Point: 6.4761

>AT106668 WP_064226358.1 NZ_CP029747:c68904-68536 [Escherichia coli]
VSDTLPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLTDRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPHHQHTVTLYAKGLTCEADTFGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT106668 NZ_CP029747:c68904-68536 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTACAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCACCATCAGCATACCGTGACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCTTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: S1EZ92