Detailed information of TA system TA106644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4669110..4669696
• Replicon: chromosome
• Accession: NZ_CP029745
• Organism: Pseudomonas aeruginosa strain AR_0110

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: AM489_RS22175
• Protein ID: WP_003120987.1
• Coordinates: 4669397..4669696 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: AM489_RS22170
• Protein ID: WP_003448662.1
• Coordinates: 4669110..4669400 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM489_RS22155	4664691..4666580	+	1890	WP_023093670.1	hypothetical protein	-
AM489_RS22160	4666577..4668553	+	1977	WP_023093671.1	DEAD/DEAH box helicase	-
AM489_RS22165	4668692..4669027	+	336	WP_022579623.1	hypothetical protein	-
AM489_RS22170	4669110..4669400	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
AM489_RS22175	4669397..4669696	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM489_RS22180	4669898..4671022	+	1125	WP_024007699.1	TcpQ domain-containing protein	-
AM489_RS22185	4671022..4672731	+	1710	WP_003123331.1	PilN family type IVB pilus formation outer membrane protein	-
AM489_RS22190	4672735..4674060	+	1326	WP_022579625.1	type 4b pilus protein PilO2	-
AM489_RS22195	4674050..4674583	+	534	WP_003149521.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4637689..4726795	89106

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T106644 WP_003120987.1 NZ_CP029745:c4669696-4669397 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T106644 NZ_CP029745:c4669696-4669397 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT106644 WP_003448662.1 NZ_CP029745:c4669400-4669110 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT106644 NZ_CP029745:c4669400-4669110 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
CTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCTCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCGACATTGAAAACCATGCTGGCA
GTAATGCGAGCTTTGGGCGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73