Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 120468..120993 Replicon plasmid unnamed1
Accession NZ_CP029742
Organism Escherichia coli strain AR_0085

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag AM464_RS28060 Protein ID WP_001159871.1
Coordinates 120688..120993 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag AM464_RS28055 Protein ID WP_000813630.1
Coordinates 120468..120686 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM464_RS28020 116149..116739 - 591 WP_032353705.1 hypothetical protein -
AM464_RS30365 116736..116996 - 261 WP_032353703.1 hypothetical protein -
AM464_RS28030 117034..118247 + 1214 WP_164473236.1 IS3 family transposase -
AM464_RS28035 118638..119090 + 453 WP_032353631.1 N-acetyltransferase -
AM464_RS28040 119122..119307 - 186 WP_032353630.1 hypothetical protein -
AM464_RS28045 119355..119486 - 132 Protein_137 transposase -
AM464_RS28055 120468..120686 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AM464_RS28060 120688..120993 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
AM464_RS28065 120994..121803 + 810 WP_045145707.1 site-specific integrase -
AM464_RS28070 121976..122329 + 354 WP_000864812.1 colicin M immunity protein -
AM464_RS28075 122379..123194 - 816 WP_000449473.1 lipid II-degrading bacteriocin colicin M -
AM464_RS28085 123318..124526 + 1209 WP_064149665.1 IS256-like element IS1414 family transposase -
AM464_RS28095 124759..125286 + 528 WP_032353548.1 colicin B immunity protein -
AM464_RS28100 125304..125963 - 660 Protein_145 colicin-B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..139740 139740
- inside IScluster/Tn - - 108186..124526 16340


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T106632 WP_001159871.1 NZ_CP029742:120688-120993 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T106632 NZ_CP029742:120688-120993 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT106632 WP_000813630.1 NZ_CP029742:120468-120686 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT106632 NZ_CP029742:120468-120686 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References