Detailed information of TA system TA106631

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 110373..110932
• Replicon: plasmid unnamed1
• Accession: NZ_CP029742
• Organism: Escherichia coli strain AR_0085

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: AM464_RS27990
• Protein ID: WP_000421257.1
• Coordinates: 110657..110932 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: AM464_RS27985
• Protein ID: WP_106631289.1
• Coordinates: 110373..110657 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM464_RS27955	105565..106602	-	1038	WP_032353476.1	iron ABC transporter permease	-
AM464_RS27960	106602..107600	-	999	WP_032353474.1	ABC transporter substrate-binding protein	-
AM464_RS27965	107750..107908	-	159	Protein_122	integrase core domain-containing protein	-
AM464_RS27970	108186..109190	-	1005	WP_000427623.1	IS110-like element IS4321 family transposase	-
AM464_RS27975	109282..109872	-	591	WP_000086536.1	recombinase family protein	-
AM464_RS27985	110373..110657	+	285	WP_106631289.1	ribbon-helix-helix domain-containing protein	Antitoxin
AM464_RS27990	110657..110932	+	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM464_RS27995	111071..112608	-	1538	Protein_127	IS66-like element ISEc8 family transposase	-
AM464_RS28000	112658..113005	-	348	WP_000612591.1	IS66 family insertion sequence element accessory protein TnpB	-
AM464_RS28005	113002..113382	-	381	WP_110844351.1	transposase	-
AM464_RS28010	113490..114216	+	727	Protein_130	IS256 family transposase	-
AM464_RS28015	114270..115628	-	1359	WP_032353707.1	esterase-like activity of phytase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..139740	139740
	-	inside	IScluster/Tn	-	-	108186..124526	16340

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T106631 WP_000421257.1 NZ_CP029742:110657-110932 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T106631 NZ_CP029742:110657-110932 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10530.06 Da
• Isoelectric Point: 7.2042

>AT106631 WP_106631289.1 NZ_CP029742:110373-110657 [Escherichia coli]
MKMKNNTVQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVKSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT106631 NZ_CP029742:110373-110657 [Escherichia coli]
ATGAAAATGAAAAACAATACCGTACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCAATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGAAATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: S1PMX5