Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 88336..88979 Replicon plasmid unnamed1
Accession NZ_CP029742
Organism Escherichia coli strain AR_0085

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag AM464_RS27845 Protein ID WP_001034046.1
Coordinates 88336..88752 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag AM464_RS27850 Protein ID WP_001261278.1
Coordinates 88749..88979 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM464_RS27805 83813..84178 - 366 WP_032353961.1 hypothetical protein -
AM464_RS27815 84269..84544 + 276 Protein_96 protein McbF -
AM464_RS27820 84588..85151 + 564 WP_032353959.1 pentapeptide repeat-containing protein -
AM464_RS27825 85352..86077 - 726 Protein_98 IS256-like element IS1414 family transposase -
AM464_RS27830 86213..86518 + 306 Protein_99 helix-turn-helix domain-containing protein -
AM464_RS27845 88336..88752 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
AM464_RS27850 88749..88979 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AM464_RS27855 89164..90651 + 1488 Protein_103 IncI1-type relaxase NikB -
AM464_RS27860 91110..91778 + 669 WP_032353458.1 cysteine hydrolase -
AM464_RS27865 91784..92644 + 861 WP_001442105.1 DUF2877 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..139740 139740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T106630 WP_001034046.1 NZ_CP029742:c88752-88336 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T106630 NZ_CP029742:c88752-88336 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATAGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT106630 WP_001261278.1 NZ_CP029742:c88979-88749 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT106630 NZ_CP029742:c88979-88749 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References