Detailed information of TA system TA106603

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2209936..2210771
• Replicon: chromosome
• Accession: NZ_CP029741
• Organism: Escherichia coli strain AR_0085

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: F4TIK8
• Locus tag: AM464_RS11500
• Protein ID: WP_000854694.1
• Coordinates: 2210394..2210771 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2U9LH69
• Locus tag: AM464_RS11495
• Protein ID: WP_074463760.1
• Coordinates: 2209936..2210304 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM464_RS11455	2205858..2206538	+	681	Protein_2110	WYL domain-containing protein	-
AM464_RS11460	2206686..2207363	+	678	WP_001097305.1	hypothetical protein	-
AM464_RS11465	2207369..2207602	+	234	WP_001278287.1	DUF905 family protein	-
AM464_RS11475	2207701..2208519	+	819	WP_001234631.1	DUF945 domain-containing protein	-
AM464_RS11480	2208601..2209080	+	480	WP_000860084.1	antirestriction protein	-
AM464_RS11485	2209096..2209572	+	477	WP_040089737.1	RadC family protein	-
AM464_RS11490	2209635..2209856	+	222	WP_001405861.1	DUF987 domain-containing protein	-
AM464_RS11495	2209936..2210304	+	369	WP_074463760.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AM464_RS11500	2210394..2210771	+	378	WP_000854694.1	TA system toxin CbtA family protein	Toxin
AM464_RS11505	2210768..2210917	+	150	Protein_2119	hypothetical protein	-
AM464_RS11510	2210993..2211190	+	198	WP_086795284.1	DUF957 domain-containing protein	-
AM464_RS11515	2211275..2212117	+	843	WP_001696593.1	DUF4942 domain-containing protein	-
AM464_RS11530	2212913..2214097	+	1185	WP_001172882.1	sugar efflux transporter	-
AM464_RS11535	2214208..2215131	+	924	WP_000535961.1	carboxylate/amino acid/amine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14008.02 Da
• Isoelectric Point: 7.7293

>T106603 WP_000854694.1 NZ_CP029741:2210394-2210771 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYGLALNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T106603 NZ_CP029741:2210394-2210771 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTCGCGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCGGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13626.39 Da
• Isoelectric Point: 6.3159

>AT106603 WP_074463760.1 NZ_CP029741:2209936-2210304 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT106603 NZ_CP029741:2209936-2210304 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1M2F934

Antitoxin

• AlphaFold DB: A0A2U9LH69