Detailed information of TA system TA106595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 58240..58883
• Replicon: plasmid unnamed2
• Accession: NZ_CP029740
• Organism: Klebsiella pneumoniae strain AR_0087

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: AM466_RS28520
• Protein ID: WP_001044770.1
• Coordinates: 58467..58883 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: AM466_RS28515
• Protein ID: WP_001261282.1
• Coordinates: 58240..58470 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM466_RS30030	53445..53747	+	303	WP_071571079.1	hypothetical protein	-
AM466_RS28475	54203..54997	-	795	WP_004197635.1	site-specific integrase	-
AM466_RS28480	55195..56211	-	1017	WP_017899884.1	hypothetical protein	-
AM466_RS28485	56222..56536	-	315	WP_053389906.1	hypothetical protein	-
AM466_RS28490	56563..56958	-	396	WP_017899885.1	hypothetical protein	-
AM466_RS28495	57127..57432	-	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	-
AM466_RS28500	57434..57652	-	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	-
AM466_RS28510	57822..58283	-	462	Protein_79	hypothetical protein	-
AM466_RS28515	58240..58470	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AM466_RS28520	58467..58883	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
AM466_RS28525	58957..59667	+	711	WP_004152334.1	ATP-binding protein	-
AM466_RS28530	59701..60398	+	698	Protein_83	IS1 family transposase	-
AM466_RS28535	60399..61247	+	849	WP_048322264.1	SDR family oxidoreductase	-
AM466_RS28540	61339..61983	-	645	WP_014386481.1	quinolone resistance pentapeptide repeat protein QnrB1	-
AM466_RS28545	62154..62744	+	591	WP_012477564.1	plasmid pRiA4b ORF-3 family protein	-
AM466_RS28550	62881..63453	+	573	WP_001493762.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrB1 / blaSHV-12 / tet(A)	-	1..103250	103250

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T106595 WP_001044770.1 NZ_CP029740:58467-58883 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T106595 NZ_CP029740:58467-58883 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT106595 WP_001261282.1 NZ_CP029740:58240-58470 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT106595 NZ_CP029740:58240-58470 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3